闪烁体的特性。来自海洋甲藻多面膝沟藻的生物发光颗粒。
The characterization of scintillons. Bioluminescent particles from the marine dinoflagellate, Gonyaulax polyedra.
作者信息
DeSa R, Hastings J W
出版信息
J Gen Physiol. 1968 Jan;51(1):105-22. doi: 10.1085/jgp.51.1.105.
A new type of biological particle, isolated from the marine dinoflagellate Gonyaulax polyedra, has been partially purified and characterized. When the pH is lowered, the particle emits light in vitro in a fashion closely mimicking the flash of the living cell, and it is referred to as a scintillon (flashing unit). Scintillons are obtained by breaking the cells in buffer at pH 8.2 and purifying by differential and sucrose density gradient centrifugation. The particle has a density of about 1.23 g cc(-1), and activity is quantitatively correlated with the number of crystal-like birhombohedral structures. These have been found to contain guanine, but since the density of authentic guanine is about 1.73 g cc(-1), the scintillon is believed to comprise additional but as yet unidentified components. The properties of the scintillon and the effects of various physical and chemical treatments are described. The reasons for believing that this particle is responsible for the flash of the intact cell are discussed.
一种从海洋双鞭毛藻多面膝沟藻中分离出的新型生物粒子已得到部分纯化和表征。当pH值降低时，该粒子在体外发光，其方式与活细胞的闪光极为相似，被称为闪烁体（闪光单位）。通过在pH 8.2的缓冲液中破碎细胞并经差速离心和蔗糖密度梯度离心进行纯化可获得闪烁体。该粒子的密度约为1.23 g·cc⁻¹，其活性与晶体状双菱面体结构的数量呈定量相关。已发现这些结构含有鸟嘌呤，但由于纯鸟嘌呤的密度约为1.73 g·cc⁻¹，因此认为闪烁体还包含其他尚未鉴定的成分。本文描述了闪烁体的性质以及各种物理和化学处理的效果。讨论了认为该粒子是完整细胞闪光原因的依据。
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