Fogel M, Hastings J W
Proc Natl Acad Sci U S A. 1972 Mar;69(3):690-3. doi: 10.1073/pnas.69.3.690.
Subcellular particles, termed scintillons, isolated from the luminescent dinoflagellate Gonyaulax, emit light when the pH is lowered from 8 to 5.7. The capacity to emit a second flash is regained by incubation of scintillons with the low molecular weight luciferin from Gonyaulax. This compound was previously demonstrated to be the substrate in the bioluminescent reaction catalyzed by a soluble luciferase isolated from the same cell. A model of the scintillon is presented that involves soluble elements structured within a membrane-bound particle.
从发光甲藻多甲藻中分离出的亚细胞颗粒,称为闪烁体,当pH值从8降至5.7时会发光。通过将闪烁体与来自多甲藻的低分子量荧光素一起孵育,可以恢复发出第二次闪光的能力。该化合物先前已被证明是由从同一细胞中分离出的可溶性荧光素酶催化的生物发光反应中的底物。本文提出了一种闪烁体模型,该模型涉及膜结合颗粒内构建的可溶性元件。