家兔内分泌胰腺A细胞和D细胞在体内及体外培养时的精细结构。I. A细胞的分泌机制。
Fine structure of the A and D cells of the rabbit endocrine pancreas in vivo and incubated in vitro. I. Mechanism of secretion of the A cells.
作者信息
Gomez-Acebo J, Parrilla R, Candela J L
出版信息
J Cell Biol. 1968 Jan;36(1):33-44. doi: 10.1083/jcb.36.1.33.
Abstract
The cytological changes observed in the A and D cells of rabbit endocrine pancreas incubated in a medium containing 0.6 mg/ml or 3 mg/ml of glucose are described. These cells showed no changes in their fine structure nor any signs of degranulation. When the A cells were incubated in a medium without glucose, they released A granules and synthesized new hormone. The way in which A granules are eliminated is compared to that following insulinic hypoglycemia in the animal in vivo. In both cases, the mechanism of secretion involves margination, emiocytosis of the entire granule, and formation of microvilli, in contrast to previously reported observations (9). The D cells showed no alteration of their fine structure after incubation with different concentrations of glucose in the medium. Only very rarely could we observe morphological changes which were suggestive of emiocytosis of the entire D granule.
摘要
描述了在含有0.6毫克/毫升或3毫克/毫升葡萄糖的培养基中孵育的兔内分泌胰腺A细胞和D细胞中观察到的细胞学变化。这些细胞的精细结构没有变化，也没有脱颗粒的迹象。当A细胞在无葡萄糖的培养基中孵育时，它们释放A颗粒并合成新的激素。将A颗粒的消除方式与动物体内胰岛素性低血糖后的情况进行了比较。在这两种情况下，分泌机制都涉及边缘化、整个颗粒的胞吐作用以及微绒毛的形成，这与之前报道的观察结果（9）不同。在培养基中用不同浓度的葡萄糖孵育后，D细胞的精细结构没有改变。我们仅能非常罕见地观察到提示整个D颗粒胞吐作用的形态学变化。
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J Cell Biol. 1968 Jan;36(1):33-44. doi: 10.1083/jcb.36.1.33.
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