Fabrikant J I
J Cell Biol. 1968 Mar;36(3):551-65. doi: 10.1083/jcb.36.3.551.
The study concerns the kinetics of cellular proliferation in the different cell populations of the normal and regenerating rat liver. A detailed analysis is presented, which includes techniques of in vivo labeling of DNA with tritiated thymidine and high-resolution radioautography, of the temporal and spatial patterns of DNA synthesis and cell division in the parenchymal cells, littoral cells, bile duct epithelium, and other cellular components in the liver during the first 64 hr of regeneration after partial hepatectomy. The analysis of cell population kinetics indicates that (a) the rate of entry of parenchymal cells into synthesis, after an initial burst of proliferative activity, was an orderly progression at 3-4%/hr; (b) most cells divided once and a few twice, a large proportion of the cell deficit being replaced by 72 hr after the onset of proliferation; (c) T(s) was approximately 8.0 hr; T(gg2+m/2), 3.0 hr; and M, approximately 1.0 hr. Littoral cell proliferation began about 24 hr after the onset of parenchymal cell proliferation; the rate of entry of littoral cells into synthesis was greater than 4%/hr. Interlobular bile duct cell proliferation lagged well behind the parenchymal and littoral cell populations both in time and extent of proliferation.
该研究涉及正常和再生大鼠肝脏不同细胞群体中的细胞增殖动力学。本文进行了详细分析，包括用氚标记的胸腺嘧啶核苷对DNA进行体内标记以及高分辨率放射自显影等技术，以研究部分肝切除术后再生的前64小时内，肝实质细胞、边缘细胞、胆管上皮细胞及肝脏其他细胞成分中DNA合成和细胞分裂的时间和空间模式。细胞群体动力学分析表明：（a）在最初一阵增殖活动之后，实质细胞进入合成期的速率为每小时3%-4%，呈有序进展；（b）大多数细胞分裂一次，少数细胞分裂两次，增殖开始后72小时，大部分细胞缺失得到补充；（c）S期约为8.0小时；G2+M期为3.0小时；M期约为1.0小时。边缘细胞增殖在实质细胞增殖开始约24小时后启动；边缘细胞进入合成期的速率大于每小时4%。小叶间胆管细胞增殖在时间和增殖程度上均远远落后于实质细胞和边缘细胞群体。
