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Inhibition of friend murine leukemia virus production by low-ionic-strength medium.

作者信息

Ussery M A, Ramirez-Mitchell R, Hardesty B A

出版信息

J Virol. 1976 Feb;17(2):453-61. doi: 10.1128/JVI.17.2.453-461.1976.

DOI:10.1128/JVI.17.2.453-461.1976
PMID:56456
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC515437/
Abstract

The effect of medium of low ionic strength on the release of virus from Friend leukemia cells has been studied. The release of infectious Friend leukemia virus is almost completely inhibited in medium of low ionic strength, as measured by a focus-forming assay (XC assay), by endogenous RNA-dependent DNA polymerase activity of released virus particles, and by electron microscope studies of the production of C-type particles. Friend leukemia virus-transformed proerythroblasts undergo extensive morphological changes in low-ionic-strength medium. The cells are viable in this medium, but they can no longer be stimulated with dimethyl sulfoxide to produce hemoglobin and increase virus production. Infectious virus is released between 30 and 120 min of resuspension of inhibited cells in normal medium. The rate of virus release after reversal of the inhibition is much greater than the rate of virus release during normal cell growth. The morphological changes occurring after dimethyl sulfoxide stimulation of Friend leukemia cells are compared with those resulting from resuspension in normal medium of cells inhibited by low ionic strength.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e50/515437/d9e2452ca383/jvirol00218-0175-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e50/515437/3687b2fafb4a/jvirol00218-0171-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e50/515437/f915bcf509b8/jvirol00218-0171-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e50/515437/42f24a3f5233/jvirol00218-0172-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e50/515437/1dadf153a833/jvirol00218-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e50/515437/119bffc7369b/jvirol00218-0174-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e50/515437/3ebab9f119f7/jvirol00218-0174-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e50/515437/b8ef8074c380/jvirol00218-0175-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e50/515437/d9e2452ca383/jvirol00218-0175-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e50/515437/3687b2fafb4a/jvirol00218-0171-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e50/515437/f915bcf509b8/jvirol00218-0171-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e50/515437/42f24a3f5233/jvirol00218-0172-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e50/515437/1dadf153a833/jvirol00218-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e50/515437/119bffc7369b/jvirol00218-0174-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e50/515437/3ebab9f119f7/jvirol00218-0174-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e50/515437/b8ef8074c380/jvirol00218-0175-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e50/515437/d9e2452ca383/jvirol00218-0175-b.jpg

相似文献

1
Inhibition of friend murine leukemia virus production by low-ionic-strength medium.
J Virol. 1976 Feb;17(2):453-61. doi: 10.1128/JVI.17.2.453-461.1976.
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Production of Friend leukaemia antigens in chronically-infected cells treated with interferon.
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Inhibition of dimethyl sulfoxide-stimulated Friend cell erythrodifferentiation by hydrocortisone and other steroids.氢化可的松及其他类固醇对二甲基亚砜刺激的弗氏细胞红系分化的抑制作用。
Proc Natl Acad Sci U S A. 1978 Aug;75(8):3851-5. doi: 10.1073/pnas.75.8.3851.

引用本文的文献

1
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Arch Virol. 1986;88(3-4):175-87. doi: 10.1007/BF01310873.
2
Effect of interferon on dimethyl sulfoxide-stimulated Friend erythroleukemic cells: ultrastructural and biochemical study.干扰素对二甲基亚砜刺激的弗氏红白血病细胞的影响:超微结构与生化研究
J Virol. 1977 Sep;23(3):799-810. doi: 10.1128/JVI.23.3.799-810.1977.
3

本文引用的文献

1
PLASTIC EMBEDDING MIXTURES FOR USE IN ELECTRON MICROSCOPY.用于电子显微镜检查的塑料包埋混合物
Stain Technol. 1964 Mar;39:111-4.
2
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中,将高pH值的柠檬酸铅用作电子不透明染色剂。
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Replication of Semliki Forest virus: an electron microscopic study.Semliki森林病毒的复制:一项电子显微镜研究。
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弗瑞德红白血病细胞系中的病毒蛋白合成
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Properties of mouse leukemia viruses. VI. The core of Friend virus; isolation and constituents.小鼠白血病病毒的特性。VI. 弗瑞德病毒的核心;分离与组成成分
Virology. 1973 Jun;53(2):457-62. doi: 10.1016/0042-6822(73)90225-0.
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Inhibition of Sindbis virus release by media of low ionic strength: an electron microscope study.低离子强度培养基对辛德毕斯病毒释放的抑制作用:一项电子显微镜研究。
J Virol. 1972 Sep;10(3):537-44. doi: 10.1128/JVI.10.3.537-544.1972.
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The effect of erythropoietin on colonial growth of erythroid precursor cells in vitro.促红细胞生成素对红系前体细胞体外集落生长的影响。
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J Natl Cancer Inst. 1973 Sep;51(3):865-74. doi: 10.1093/jnci/51.3.865.