Cooper M C, Levy J, Cantor L N, Marks P A, Rifkind R A
Proc Natl Acad Sci U S A. 1974 May;71(5):1677-80. doi: 10.1073/pnas.71.5.1677.
A method is described for the colonial growth, in semi-solid medium, of erythropoietin-responsive erythroid cell precursors. The erythroid cell precursors were isolated by immune hemolysis from fetal mouse liver. Both the number of precursor cells triggered to proliferate and differentiate, and the size of the erythropoietic colonies formed, are directly dependent upon the concentration of erythropoietin included in the culture.
本文描述了一种在半固体培养基中使促红细胞生成素反应性红系细胞前体进行集落生长的方法。通过免疫溶血从胎鼠肝脏中分离出红系细胞前体。被触发增殖和分化的前体细胞数量以及所形成的造血集落大小，都直接取决于培养物中促红细胞生成素的浓度。
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