Cohen S, Sweeney H M
J Bacteriol. 1968 Apr;95(4):1368-74. doi: 10.1128/jb.95.4.1368-1374.1968.
Previously described penicillinase-constitutive mutations in Staphylococcus aureus are caused by genetic lesions in a regulator gene (or genes) on the penicillinase plasmid in close linkage to the structural gene. This report describes a new class (R2(-)) of penicillinase-constitutive mutants of S. aureus unlinked to the plasmid. By transductional analysis, the penicillinase plasmids in these mutants were wild type. Wild-type plasmids transduced into penicillinase-negative (plasmid loss) derivatives of R2(-) mutants produced penicillinase constitutively in amounts comparable to a fully induced culture of the wild-type strain. Penicillinase production in R2(-) mutants was maximal at 30 to 32 C and was much reduced at 40 C.
先前描述的金黄色葡萄球菌青霉素酶组成型突变是由青霉素酶质粒上与结构基因紧密连锁的一个或多个调节基因中的遗传损伤引起的。本报告描述了一类新的（R2(-)）与质粒不连锁的金黄色葡萄球菌青霉素酶组成型突变体。通过转导分析，这些突变体中的青霉素酶质粒是野生型的。导入R2(-)突变体的青霉素酶阴性（质粒丢失）衍生物中的野生型质粒组成型地产生青霉素酶，其产量与野生型菌株的完全诱导培养物相当。R2(-)突变体中的青霉素酶产量在30至32℃时最高，在40℃时则大大降低。
