Fox J B, Holtman D F
J Bacteriol. 1968 May;95(5):1548-50. doi: 10.1128/jb.95.5.1548-1550.1968.
Five strains of Staphylococcus aureus were examined quantitatively for the production of nuclease under aerobic and anaerobic conditions. Hydrolysis of deoxyribonucleic acid and ribonucleic acid was detected by measuring the release of acidsoluble nucleotides spectrophotometrically. We found that the enzyme was produced anaerobically, as well as aerobically, and that anaerobiosis had no effect on production of this enzyme when other conditions, such as pH, were held constant.
对五株金黄色葡萄球菌在需氧和厌氧条件下核酸酶的产生进行了定量检测。通过分光光度法测量酸溶性核苷酸的释放来检测脱氧核糖核酸和核糖核酸的水解。我们发现,该酶在需氧和厌氧条件下均能产生,并且当其他条件(如pH值)保持恒定时,厌氧对该酶的产生没有影响。