红螺菌中丙酸作为内源性二氧化碳受体的鉴定及纯化的丙酰辅酶A羧化酶的性质
Identification of propionate as an endogenous CO2 acceptor in Rhodospirillum rubrum and properties of purified propionyl-coenzyme A carboxylase.
作者信息
Olsen I, Merrick J M
出版信息
J Bacteriol. 1968 May;95(5):1774-8. doi: 10.1128/jb.95.5.1774-1778.1968.
Abstract
A heat-stable endogenous CO(2) acceptor has been found in extracts of Rhodospirillum rubrum grown photoheterotrophically on acetate. Evidence is presented which suggests that this factor is propionic acid. Thus, paper and gas chromatographic analyses have indicated that propionic acid is present in boiled extracts prepared from R. rubrum cells. The products of (14)CO(2) fixation obtained with either the boiled extract or propionic acid as the CO(2) acceptor were identical and were identified as methylmalonic acid and succinic acid by paper chromatography. The enzyme which catalyzes the carboxylation of propionyl-coenzyme A (propionyl-CoA carboxylase) was purified from R. rubrum cells grown on acetate and its properties were studied. The enzyme is similar to propionyl-CoA carboxylases isolated from mammalian sources.
摘要
在以醋酸盐为光异养生长的深红红螺菌提取物中发现了一种热稳定的内源性二氧化碳受体。有证据表明该因子是丙酸。因此，纸色谱和气相色谱分析表明，丙酸存在于从深红红螺菌细胞制备的煮沸提取物中。以煮沸提取物或丙酸作为二氧化碳受体获得的二氧化碳固定产物是相同的，通过纸色谱鉴定为甲基丙二酸和琥珀酸。从以醋酸盐生长的深红红螺菌细胞中纯化了催化丙酰辅酶A羧化的酶（丙酰辅酶A羧化酶）并研究了其性质。该酶类似于从哺乳动物来源分离的丙酰辅酶A羧化酶。
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