乙烯的生物合成。参与由甲硫醛形成乙烯的酶。
Biosynthesis of ethylene. Enzymes involved in its formation from methional.
作者信息
Mapson L W, Wardale D A
出版信息
Biochem J. 1968 Apr;107(3):433-42. doi: 10.1042/bj1070433.
Abstract
- Two enzymes were shown to be necessary for the production of ethylene from methional; they were separated from extracts of cauliflower florets by fractionation on Sephadex and other methods. 2. The first enzyme, generating hydrogen peroxide, appears to be similar to the fungal glucose oxidase, for like the latter it is highly specific for its substrate d-glucose. 3. The second enzyme, in the presence of cofactors and peroxide generated by the first enzyme, cleaves methional to ethylene. 4. It was also found that hydrogen peroxide in these reactions may be replaced by hydroperoxide generated from linolenic acid by lipoxidase enzymes. 5. Dihydroxyphenols were shown to have a marked inhibitory effect on these reactions and to account for the initial phase of low activity that is always observed in aqueous extracts prepared from the floret tissue.
摘要
- 已证明从甲硫醛生成乙烯需要两种酶；通过在葡聚糖凝胶上分级分离及其他方法，将它们从花椰菜小花提取物中分离出来。2. 第一种酶产生过氧化氢，似乎与真菌葡萄糖氧化酶相似，因为与后者一样，它对其底物d -葡萄糖具有高度特异性。3. 第二种酶在辅因子和第一种酶产生的过氧化物存在下，将甲硫醛裂解为乙烯。4. 还发现这些反应中的过氧化氢可用脂氧化酶从亚麻酸生成的氢过氧化物替代。5. 已表明二羟基酚对这些反应有显著抑制作用，并解释了从小花组织制备的水提取物中始终观察到的低活性初始阶段。
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本文引用的文献
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Ethylene production from methionine.由甲硫氨酸产生乙烯。
Biochem J. 1965 Nov;97(2):449-59. doi: 10.1042/bj0970449.
2
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
4
Biosynthesis of ethylene. Ethylene formation from methional by horseradish peroxidase.乙烯的生物合成。辣根过氧化物酶催化甲硫醛生成乙烯。
Arch Biochem Biophys. 1967 Nov;122(2):481-7. doi: 10.1016/0003-9861(67)90222-6.
5
Biosynthesis of ethylene. Formation of ethylene from methional by a cell-free enzyme system from cauliflower florets.乙烯的生物合成。通过来自花椰菜小花的无细胞酶系统由甲硫醛形成乙烯。
Biochem J. 1967 Feb;102(2):574-85. doi: 10.1042/bj1020574.
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Ethylene production from methionine as mediated by flavin mononucleotide and light.由甲硫氨酸经黄素单核苷酸和光介导产生乙烯。
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