乙烯的生物合成。通过来自花椰菜小花的无细胞酶系统由甲硫醛形成乙烯。
Biosynthesis of ethylene. Formation of ethylene from methional by a cell-free enzyme system from cauliflower florets.
作者信息
Mapson L W, Wardale D A
出版信息
Biochem J. 1967 Feb;102(2):574-85. doi: 10.1042/bj1020574.
Abstract
- The formation of ethylene from cauliflower florets is stimulated by the addition of either methionine or its hydroxy analogue. 2. Formation of ethylene from these compounds may also be demonstrated in cell-free extracts, but the most rapid formation is achieved by the addition of methional. 3. Fractionation of such extracts has shown that both particulate and non-particulate fractions are necessary for the formation of ethylene from methionine or its hydroxy analogues, but only the non-particulate fraction is necessary for its formation from methional. 4. A study of this system has shown that the conversion of methional into ethylene requires the presence of two enzyme systems, the first generating peroxide and the second catalysing the conversion of methional into ethylene in the presence of peroxide. 5. The presence of a heat-stable factor in cauliflower extracts that is necessary for the full activity of the enzyme converting methional into ethylene has also been shown. 6. The nature of this factor is at the present unknown; it is not a metal nor is it identifiable with many of the known coenzymes.
摘要
- 向花椰菜小花中添加甲硫氨酸或其羟基类似物会刺激乙烯的形成。2. 从这些化合物中形成乙烯在无细胞提取物中也能得到证明，但通过添加甲硫醛能实现最快速的形成。3. 对这类提取物进行分级分离表明，从甲硫氨酸或其羟基类似物形成乙烯时，颗粒部分和非颗粒部分都是必需的，但从甲硫醛形成乙烯时仅非颗粒部分是必需的。4. 对该系统的一项研究表明，甲硫醛转化为乙烯需要两种酶系统，第一种产生过氧化物，第二种在过氧化物存在的情况下催化甲硫醛转化为乙烯。5. 还表明花椰菜提取物中存在一种热稳定因子，它对于将甲硫醛转化为乙烯的酶的充分活性是必需的。6. 目前该因子的性质尚不清楚；它不是一种金属，也无法与许多已知的辅酶相鉴别。
相似文献
1
Biosynthesis of ethylene. Formation of ethylene from methional by a cell-free enzyme system from cauliflower florets.乙烯的生物合成。通过来自花椰菜小花的无细胞酶系统由甲硫醛形成乙烯。
Biochem J. 1967 Feb;102(2):574-85. doi: 10.1042/bj1020574.
2
Biosynthesis of ethylene. Enzymes involved in its formation from methional.乙烯的生物合成。参与由甲硫醛形成乙烯的酶。
Biochem J. 1968 Apr;107(3):433-42. doi: 10.1042/bj1070433.
3
Biosynthesis of ethylene. Dual nature of cofactor required for the enzymic production of ethylene from methional.乙烯的生物合成。从甲硫醛酶促生成乙烯所需辅因子的双重性质。
Biochem J. 1968 Aug;108(5):875-81. doi: 10.1042/bj1080875.
4
Biosynthesis of ethylene. Ethylene formation from methional by horseradish peroxidase.乙烯的生物合成。辣根过氧化物酶催化甲硫醛生成乙烯。
Arch Biochem Biophys. 1967 Nov;122(2):481-7. doi: 10.1016/0003-9861(67)90222-6.
5
Biosynthesis of ethylene. Methanesulphinic acid as cofactor in the enzymic formation of ethylene from methional.乙烯的生物合成。甲硫氨酸作为从甲硫醛酶促形成乙烯过程中的辅助因子。
Biochem J. 1969 Feb;111(4):413-8. doi: 10.1042/bj1110413.
6
Enzymic evolution of ethylene from methional by a pea seedling extract.豌豆幼苗提取物将甲硫醛转化为乙烯的酶促演化过程。
Arch Biochem Biophys. 1967 Mar 20;118(3):756-8. doi: 10.1016/0003-9861(67)90413-4.
7
Evaluation of the role of methional, 2-keto-4-methylthiobutyric acid and peroxidase in ethylene formation by Escherichia coli.甲硫醛、2-酮-4-甲基硫代丁酸和过氧化物酶在大肠杆菌乙烯形成中的作用评估。
J Gen Microbiol. 1977 Feb;98(2):519-28. doi: 10.1099/00221287-98-2-519.
8
Biosynthesis of ethylene. 4-methylmercapto-2-oxobutyric acid: an intermediate in the formation from methionine.乙烯的生物合成。4-甲硫基-2-氧代丁酸：由甲硫氨酸形成过程中的一种中间体。
Biochem J. 1969 Dec;115(4):653-61. doi: 10.1042/bj1150653.
9
Lactoferrin-catalysed hydroxyl radical production. Additional requirement for a chelating agent.乳铁蛋白催化的羟基自由基生成。对螯合剂的额外需求。
Biochem J. 1983 Jan 15;210(1):15-9. doi: 10.1042/bj2100015.
10
3-methylthiopropionaldehyde peroxidase from apples: an ethylene-forming enzyme.苹果中的3-甲基硫代丙醛过氧化物酶：一种乙烯形成酶。
Biochim Biophys Acta. 1969 Apr 22;178(2):235-47. doi: 10.1016/0005-2744(69)90393-3.
引用本文的文献
1
An evaluation of 4-s-methyl-2-keto-butyric Acid as an intermediate in the biosynthesis of ethylene.对4-甲基-2-氧代丁酸作为乙烯生物合成中间体的评估。
Plant Physiol. 1971 Apr;47(4):576-80. doi: 10.1104/pp.47.4.576.
2
Mechanism of Auxin-induced Ethylene Production.生长素诱导乙烯生成的机制。
Plant Physiol. 1971 Apr;47(4):504-9. doi: 10.1104/pp.47.4.504.
3
Precursors of ethylene.乙烯的前体。
Plant Physiol. 1969 Sep;44(9):1347-9. doi: 10.1104/pp.44.9.1347.
4
beta-Alanine as an Ethylene Precursor. Investigations Towards Preparation, and Properties, of a Soluble Enzyme System From a Subcellular Particulate Fraction of Bean Cotyledons.β-丙氨酸作为乙烯前体。从菜豆子叶的亚细胞颗粒部分制备和研究一种可溶性酶体系。
Plant Physiol. 1969 Sep;44(9):1217-26. doi: 10.1104/pp.44.9.1217.
5
Biosynthesis of ethylene. Methanesulphinic acid as cofactor in the enzymic formation of ethylene from methional.乙烯的生物合成。甲硫氨酸作为从甲硫醛酶促形成乙烯过程中的辅助因子。
Biochem J. 1969 Feb;111(4):413-8. doi: 10.1042/bj1110413.
6
Biosynthesis of ethylene. Dual nature of cofactor required for the enzymic production of ethylene from methional.乙烯的生物合成。从甲硫醛酶促生成乙烯所需辅因子的双重性质。
Biochem J. 1968 Aug;108(5):875-81. doi: 10.1042/bj1080875.
7
Biosynthesis of ethylene. Enzymes involved in its formation from methional.乙烯的生物合成。参与由甲硫醛形成乙烯的酶。
Biochem J. 1968 Apr;107(3):433-42. doi: 10.1042/bj1070433.
8
Stimulation by Erwinia carotovora of the synthesis of ethylene in cauliflower tissue.胡萝卜软腐欧文氏菌对花椰菜组织中乙烯合成的刺激作用。
Biochem J. 1970 Sep;119(2):251-63. doi: 10.1042/bj1190251.
9
A comparative study of the ability of methionine or linolenic acid to act as precursors of ethylene in plant tissues.蛋氨酸或亚麻酸作为植物组织中乙烯前体能力的比较研究。
Biochem J. 1970 Apr;117(3):473-9. doi: 10.1042/bj1170473.
10
Biosynthesis of ethylene. 4-methylmercapto-2-oxobutyric acid: an intermediate in the formation from methionine.乙烯的生物合成。4-甲硫基-2-氧代丁酸：由甲硫氨酸形成过程中的一种中间体。
Biochem J. 1969 Dec;115(4):653-61. doi: 10.1042/bj1150653.
本文引用的文献
1
Ethylene production from methionine.由甲硫氨酸产生乙烯。
Biochem J. 1965 Nov;97(2):449-59. doi: 10.1042/bj0970449.
2
Stimulation of ethylene production in apple tissue slices by methionine.甲硫氨酸刺激苹果组织切片中乙烯的产生。
Plant Physiol. 1966 Mar;41(3):376-82. doi: 10.1104/pp.41.3.376.
3
HISTOLOGICAL REACTIONS OF BUFO MELANOSTICTUS TO CULTURE FILTRATES AND MYCELIAL EXTRACTS OF A PATHOGENIC FUNGUS.黑眶蟾蜍对一种致病真菌的培养滤液和菌丝提取物的组织学反应
Nature. 1964 Oct 24;204:345-7. doi: 10.1038/204345a0.
Zotero 插件