Martin T E, Henricks D M, Hill J R, Rawlings N C
J Reprod Fertil. 1978 May;53(1):173-8. doi: 10.1530/jrf.0.0530173.
Six beef heifers were immunized over a 4-month period with an oestradiol-17beta-BSA conjugate in Freund's adjuvant. There was an interference with oestrus in the treated heifers; 2 ceased to exhibit oestrus, one exhibited one oestrus and three exhibited oestrus after Day 47 of treatment. The control heifers treated with Freund's adjuvant had normal oestrous cycles. The antiserum titre rose in all treated heifers and attained its highest level in the 2 animals in which oestrus did not recur. The temporal changes in plasma LH, progesterone and oestradiol were normal during the pretreatment period, but became abnormal during the 120 days after immunization. Although plasma oestradiol-17beta rose at the expected time of oestrus after treatment, it was apparently effectively neutralized by the antiserum induced by treatment as evidenced by the absence of an LH surge. Plasma progesterone levels fell to baseline and remained low, indicating lack of formation of corpora lutea.
六头小母牛在4个月的时间里用弗氏佐剂中的17β-雌二醇-牛血清白蛋白结合物进行免疫。处理后的小母牛出现发情干扰；2头停止表现出发情，1头表现出一次发情，3头在处理后第47天之后表现出发情。用弗氏佐剂处理的对照小母牛发情周期正常。所有处理后的小母牛抗血清滴度均升高，在未再次发情的2头动物中达到最高水平。预处理期间血浆促黄体生成素、孕酮和雌二醇的时间变化正常，但免疫后120天内变得异常。尽管处理后血浆17β-雌二醇在预期的发情时间升高，但由于促黄体生成素激增的缺失，显然被处理诱导的抗血清有效中和。血浆孕酮水平降至基线并保持在低水平，表明黄体未形成。
