Kaushansky A, Bauminger S, Koch Y, Lindner H R
Acta Endocrinol (Copenh). 1977 Apr;84(4):795-803. doi: 10.1530/acta.0.0840795.
Mature female rats were immunized with BSA-conjugates of oestradiol-17beta-6-carboxymethyloxime or progesterone-11-hymisuccinate. Sera tested two months later at 1/2000 dilution bound 63.3% +/- 3.1 SEM and 21.2% +/- 2.3 of the homologous tritiated hormone (10 pg),--oestradiol-17beta(Oe2) and progesterone (P), respectively. Rats immunized against Oe2 had a significantly longer oestrous cycle (8.2 days vs. 4.5 days in BSA-immunized rats), with a prolonged leukocytic phase. The cycle of rats immunized against P was also prolonged (10.5 days), but in this group the cornified smear phase was abnormally extended. The luteinizing hormone (LH) normally observed on the afternoon of pro-oestrus failed to occur in the rats immunized against Oe2 and in 70% of rats immunized against P. The latter animals, however, showed sporadic LH discharges during periods of persistent cornification. Immunization against P resulted in elevated total (free plus bound) plasma P levels; immunization against Oe2 prevented the pro-oestrous rise in plasma P. Mating of rats immunized against Oe2 resulated in normal pregnanices, while none of the rats immunized against P delivered.
将成熟雌性大鼠用17β - 雌二醇 - 6 - 羧甲基肟或11 - 琥珀酸孕酮的牛血清白蛋白缀合物进行免疫。两个月后，以1/2000稀释度检测的血清分别结合了63.3%±3.1标准误和21.2%±2.3的同源氚标记激素（10皮克），即17β - 雌二醇（Oe2）和孕酮（P）。用Oe2免疫的大鼠发情周期显著延长（8.2天，而牛血清白蛋白免疫的大鼠为4.5天），白细胞期延长。用P免疫的大鼠周期也延长（10.5天），但该组角化涂片期异常延长。正常情况下在发情前期下午观察到的促黄体生成素（LH）在免疫Oe2的大鼠以及70%免疫P的大鼠中未出现。然而，后一组动物在持续角化期出现了散发性LH释放。免疫P导致血浆总P水平（游离加结合）升高；免疫Oe2阻止了发情前期血浆P的升高。免疫Oe2的大鼠交配后正常怀孕，而免疫P的大鼠无一产仔。
