从正常胚胎鸡细胞基因组中释放的活跃DNA转录位点。
Active DNA transcription sites released from the genome of normal embryonic chicken cells.
作者信息
Leibovitch S A, Harel J
出版信息
Nucleic Acids Res. 1978 Mar;5(3):777-87. doi: 10.1093/nar/5.3.777.
Single-stranded DNA (ssDNA) isolated from (and amounting to 1.5-2% of) native nuclear DNA of cultured embryonic chicken cells labelled 1-2 days with 3H-thymidine was analyzed by self-hybridization, hydroxyapatite chromatography (HAC) partial digestion with S1 nuclease, isopycnic centrifugation. Two main fractions were rehybridized to excess amounts of bulk nuclear DNA or total cytoplasmic RNAs. The major fraction, equivalent to 75% of total ssDNA, consists of unique DNA sequences, apparently derived from multiple coding regions of the cell genome, since they are not self-reassociating but are hybridizable to the non repetitious portion of bulk nuclear DNA and 40-45% of them are complementary to cell RNAs. About half of these ssDNA sequences hybridizable to cell RNAs seem to be closely connected with molecules belonging to the minor ssDNA fraction. The latter fraction consists of self-reassociating, moderately repeated DNA sequences, mainly derived from non coding regions of the cell genome. These findings are discussed in the light of others, showing interspersion of coding and non coding DNA sequences and susceptibility of active genes to certain nucleasic attacks.
从用³H-胸腺嘧啶标记1 - 2天的培养胚胎鸡细胞的天然核DNA中分离出的单链DNA（ssDNA，占天然核DNA的1.5 - 2%），通过自身杂交、羟基磷灰石色谱法（HAC）、用S1核酸酶部分消化、等密度离心进行分析。将两个主要部分与过量的大量核DNA或总细胞质RNA重新杂交。主要部分相当于总ssDNA的75%，由独特的DNA序列组成，显然源自细胞基因组的多个编码区域，因为它们不会自身重新结合，但可与大量核DNA的非重复部分杂交，并且其中40 - 45%与细胞RNA互补。这些可与细胞RNA杂交的ssDNA序列中，约一半似乎与属于次要ssDNA部分的分子紧密相连。后一部分由自身重新结合、中度重复的DNA序列组成，主要源自细胞基因组的非编码区域。根据其他研究结果对这些发现进行了讨论，这些研究结果表明编码和非编码DNA序列相互穿插，以及活性基因对某些核酸攻击敏感。
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