与动物细胞转录活性相关的单链DNA的分离。
Isolation of single stranded DNA related to the transcriptional activity of animal cells.
作者信息
Tapiero H, Leibowitch S A, Shaool D, Monier M N, Harel J
出版信息
Nucleic Acids Res. 1976 Apr;3(4):953-63. doi: 10.1093/nar/3.4.953.
Abstract
Single stranded DNA (s.s.DNA) comprising 1-2% of the total nuclear DNA was isolated by an improved method of hydroxyapatite chromatography from native nuclear DNA3 of embryonic chick cells, labeled for several cell generations with 3H-thymidine. Small quantities of 3H-DNA were annealed with a large excess of unlabeled DNA or polysomal RNA from chick embryos. Hybridization kinetics (monitored by the use of SI nuclease digestion, hydroxyapatite chromatography and thermalfusion), indicated that s.s.DNA belongs to the non repetitious fraction of the cell genome. One third represents DNA sequences engaged in the transcription of messenger RNA's.
摘要
通过改进的羟基磷灰石层析法，从用³H-胸腺嘧啶标记了几代细胞的胚胎鸡细胞天然核DNA中分离出占总核DNA 1 - 2%的单链DNA（s.s.DNA）。将少量³H-DNA与大量过量的来自鸡胚胎的未标记DNA或多聚核糖体RNA进行退火。杂交动力学（通过使用S1核酸酶消化、羟基磷灰石层析和热融合进行监测）表明，s.s.DNA属于细胞基因组的非重复部分。其中三分之一代表参与信使RNA转录的DNA序列。
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