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相似文献

1
Isolation of single stranded DNA related to the transcriptional activity of animal cells.与动物细胞转录活性相关的单链DNA的分离。
Nucleic Acids Res. 1976 Apr;3(4):953-63. doi: 10.1093/nar/3.4.953.
2
Active DNA transcription sites released from the genome of normal embryonic chicken cells.从正常胚胎鸡细胞基因组中释放的活跃DNA转录位点。
Nucleic Acids Res. 1978 Mar;5(3):777-87. doi: 10.1093/nar/5.3.777.
3
Relationship between single-stranded DNA isolated from cultured muscular cells during differentiation and the transcription of messenger RNA.
Eur J Biochem. 1979 Jul;97(2):327-33. doi: 10.1111/j.1432-1033.1979.tb13118.x.
4
[Demonstration of repeated sequences of DNA complementary to polysomic messenger RNA from chicken liver].[鸡肝多倍体信使核糖核酸互补DNA重复序列的证实]
C R Acad Hebd Seances Acad Sci D. 1975 Jan 20;280(3):355-8.
5
Structure of nuclear pre-mRNA. VI. "Reversed repeats" in animal DNA and their hybridization with double-stranded regions of pre-mRNA.核内前体信使核糖核酸的结构。VI. 动物DNA中的“反向重复序列”及其与前体信使核糖核酸双链区域的杂交
Mol Biol. 1975 Jan;8(4):503-9.
6
Highly repetitive sequences of DNA in chromosomes.染色体中高度重复的DNA序列。
Int Rev Cytol. 1972;32:1-51. doi: 10.1016/s0074-7696(08)60337-x.
7
Characteristics of the rapidly reassociating deoxyribonucleic acid of Cucurbita pepo L. and the sequences complementary to ribosomal and transfer ribonucleic acids.西葫芦快速复性脱氧核糖核酸的特性以及与核糖体核糖核酸和转移核糖核酸互补的序列
Biochemistry. 1973 Jul 3;12(14):2759-65. doi: 10.1021/bi00738a032.
8
Isolation of a mouse DNA fraction which encodes more informational than non informational RNA sequences.分离出一种小鼠DNA组分，其编码的信息性RNA序列多于非信息性RNA序列。
Mol Biol Rep. 1982 Mar 31;8(2):91-6. doi: 10.1007/BF00778510.
9
A measurement of the sequence complexity of polysomal messenger RNA in sea urchin embryos.对海胆胚胎中多聚核糖体信使核糖核酸序列复杂性的测量。
Cell. 1974 May;2(1):9-20. doi: 10.1016/0092-8674(74)90003-8.
10
DNA synthesis by isolated mitochondria. IV. Isolation of an intermediate containing newly synthesized DNA in full-length light strands.分离线粒体的DNA合成。IV. 全长轻链中含有新合成DNA的中间体的分离。
Biochim Biophys Acta. 1972 Jul 20;272(3):341-9.

引用本文的文献

1
Quantitative amplification of single-stranded DNA (QAOS) demonstrates that cdc13-1 mutants generate ssDNA in a telomere to centromere direction.单链DNA定量扩增（QAOS）表明，cdc13-1突变体在端粒到着丝粒的方向上产生单链DNA。
Nucleic Acids Res. 2001 Nov 1;29(21):4414-22. doi: 10.1093/nar/29.21.4414.
2
Active DNA transcription sites released from the genome of normal embryonic chicken cells.从正常胚胎鸡细胞基因组中释放的活跃DNA转录位点。
Nucleic Acids Res. 1978 Mar;5(3):777-87. doi: 10.1093/nar/5.3.777.
3
Structural defects in rat liver deoxyribonucleic acid. Endogenous single-strained regions in comparison with damage induced in vivo by a carcinogen.大鼠肝脏脱氧核糖核酸的结构缺陷。内源性单链区域与致癌物在体内诱导的损伤的比较。
Biochem J. 1979 May 1;179(2):341-52. doi: 10.1042/bj1790341.
4
Single-stranded DNA from oncornavirus-infected cells enriched in virus-specific DNA sequences.来自感染肿瘤病毒的细胞的单链DNA富含病毒特异性DNA序列。
Proc Natl Acad Sci U S A. 1977 Sep;74(9):3720-4. doi: 10.1073/pnas.74.9.3720.

本文引用的文献

1
SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.DNA大小与形状的沉降研究
J Mol Biol. 1965 Feb;11:373-90. doi: 10.1016/s0022-2836(65)80064-x.
2
State of newly synthesized HeLa DNA.新合成的海拉细胞DNA的状态。
Nature. 1969 Mar 29;221(5187):1215-7. doi: 10.1038/2211215a0.
3
Replication of repetitious DNA and the S period.重复DNA的复制与S期。
Proc Natl Acad Sci U S A. 1970 Sep;67(1):448-55. doi: 10.1073/pnas.67.1.448.
4
A crude nuclease preparation suitable for use in DNA reassociation experiments.一种适用于DNA重缔合实验的粗制核酸酶制剂。
Biochim Biophys Acta. 1971 Jul 29;240(4):522-31. doi: 10.1016/0005-2787(71)90709-x.
5
Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains.DNA链生长的机制。I. 新合成链可能的不连续性和异常二级结构。
Proc Natl Acad Sci U S A. 1968 Feb;59(2):598-605. doi: 10.1073/pnas.59.2.598.
6
General model for the chromosomes of higher organisms.高等生物染色体的通用模型。
Nature. 1971 Nov 5;234(5323):25-7. doi: 10.1038/234025a0.
7
Breaks in DNA stimulate transcription by core RNA polymerase.DNA断裂会刺激核心RNA聚合酶进行转录。
Nature. 1969 Aug 23;223(5208):854-5. doi: 10.1038/223854a0.
8
Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.DNA中的重复序列。数以十万计的DNA序列拷贝已被纳入高等生物的基因组中。
Science. 1968 Aug 9;161(3841):529-40. doi: 10.1126/science.161.3841.529.
9
On the structure of the folded chromosome of Escherichia coli.关于大肠杆菌折叠染色体的结构
J Mol Biol. 1972 Nov 14;71(2):127-47. doi: 10.1016/0022-2836(72)90342-7.
10
Replication of repetitious DNA in synchronized chick fibroblast cells.同步化鸡成纤维细胞中重复DNA的复制
Exp Cell Res. 1974 Nov;89(1):39-46. doi: 10.1016/0014-4827(74)90184-0.

与动物细胞转录活性相关的单链DNA的分离。

Isolation of single stranded DNA related to the transcriptional activity of animal cells.

作者信息

Tapiero H, Leibowitch S A, Shaool D, Monier M N, Harel J

出版信息

Nucleic Acids Res. 1976 Apr;3(4):953-63. doi: 10.1093/nar/3.4.953.

DOI:10.1093/nar/3.4.953
PMID:944919
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342957/
Abstract

Single stranded DNA (s.s.DNA) comprising 1-2% of the total nuclear DNA was isolated by an improved method of hydroxyapatite chromatography from native nuclear DNA3 of embryonic chick cells, labeled for several cell generations with 3H-thymidine. Small quantities of 3H-DNA were annealed with a large excess of unlabeled DNA or polysomal RNA from chick embryos. Hybridization kinetics (monitored by the use of SI nuclease digestion, hydroxyapatite chromatography and thermalfusion), indicated that s.s.DNA belongs to the non repetitious fraction of the cell genome. One third represents DNA sequences engaged in the transcription of messenger RNA's.

摘要

通过改进的羟基磷灰石层析法，从用³H-胸腺嘧啶标记了几代细胞的胚胎鸡细胞天然核DNA中分离出占总核DNA 1 - 2%的单链DNA（s.s.DNA）。将少量³H-DNA与大量过量的来自鸡胚胎的未标记DNA或多聚核糖体RNA进行退火。杂交动力学（通过使用S1核酸酶消化、羟基磷灰石层析和热融合进行监测）表明，s.s.DNA属于细胞基因组的非重复部分。其中三分之一代表参与信使RNA转录的DNA序列。