鸡细胞核DNA中重复序列的分布
Distribution of repetitious sequences in chick nuclear DNA.
作者信息
Tapiero H, Monier M N, Shaool D, Harel J
出版信息
Nucleic Acids Res. 1974 Feb;1(2):309-22. doi: 10.1093/nar/1.2.309.
By an improved method of hydroxylapatite chromatography, the reassociated sequences of chick nuclear DNA were isolated, and their base composition analysed. By increasing the amount of reassociation, the G + C content of the renatured sequences decreased progressively to reach a mean value corresponding to that of the total DNA. In order to study the distribution of the families, or group of families having different amount of reassociation, DNA was fractionated by CsC1 density gradient centrifugation. Fractions having different G + C content were obtained, and their reassociation rates analysed. At high C(o)t value of renaturation (C(o)t=50) the amount of reassociated sequences included in the high or in the low buoyant density DNA fractions was approximately the same, but their G + C content was as expected different. At lower C(o)t values of renaturation (between C(o)t of 0.2 and the C(o)t of 10), the results indicated an heterogeneity of the repeated sequences in the A + T rich DNA fractions, as compared to the G + C rich ones.
通过改进的羟基磷灰石色谱法，分离出鸡细胞核DNA的重缔合序列，并分析其碱基组成。通过增加重缔合的量，复性序列的G + C含量逐渐降低，达到与总DNA相对应的平均值。为了研究具有不同重缔合程度的家族或家族组的分布，通过CsC1密度梯度离心对DNA进行分级分离。获得了具有不同G + C含量的级分，并分析了它们的重缔合率。在高复性C（o）t值（C（o）t = 50）时，高浮力密度或低浮力密度DNA级分中包含的重缔合序列的量大致相同，但它们的G + C含量如预期的那样不同。在较低的复性C（o）t值（在0.2的C（o）t和10的C（o）t之间），结果表明，与富含G + C的DNA级分相比，富含A + T的DNA级分中的重复序列存在异质性。
Zotero 插件