大豆豆血红蛋白和抹香鲸肌红蛋白的拟过氧化物酶活性研究。

Study of the pseudoperoxidatic activity of soybean leghemoglobin and sperm whale myoglobin.

作者信息

Sievers G, Rönnberg M

出版信息

Biochim Biophys Acta. 1978 Apr 26;533(2):293-301. doi: 10.1016/0005-2795(78)90376-8.

Abstract

The compound formation between soybean leghemoglobins a and c and H2O2 or ethyl hydroperoxide has been studied and compared with the hydrogen peroxide compound of sperm whale myoglobin. the titration data show that the hydrogen peroxide compounds of leghemoglobins are formed in a 1:1 molar ratio. The kinetics of the formation of the compounds follow first-order kinetics and the compounds are formed considerably faster than the myoglobin peroxide compound. The pseudoperoxidatic activity of leghemoglobins a and c and myoglobin was studied using guaiacol as electron donor. The maximal reaction velocities of leghemoglobins are greater than that of myoglobin. The results indicate that the peroxidatic activity of ferrileghemoglobin may be biologically important for instance in aging root nodules.

摘要

对大豆豆血红蛋白a和c与过氧化氢或氢过氧化乙酯之间的化合物形成进行了研究，并与抹香鲸肌红蛋白的过氧化氢化合物进行了比较。滴定数据表明，豆血红蛋白的过氧化氢化合物以1:1的摩尔比形成。化合物形成的动力学遵循一级动力学，并且这些化合物的形成速度比肌红蛋白过氧化物化合物快得多。以愈创木酚作为电子供体，研究了豆血红蛋白a和c以及肌红蛋白的拟过氧化物酶活性。豆血红蛋白的最大反应速度大于肌红蛋白。结果表明，高铁豆血红蛋白的过氧化物酶活性在生物学上可能很重要，例如在衰老的根瘤中。