DNA聚合酶与哺乳动物细胞核染色质核小体的关联。

Association of DNA polymerase with nucleosomes from mammalian cell chromatin.

作者信息

Schlaeger E J, van Telgen H J, Klempnauer K H, Knippers R

出版信息

Eur J Biochem. 1978 Mar;84(1):95-102. doi: 10.1111/j.1432-1033.1978.tb12145.x.

DOI:10.1111/j.1432-1033.1978.tb12145.x

Abstract

More than half of the DNA polymerase beta in mouse ascites cell chromatin was found to be associated with monomeric nucleosomal particles (produced by micrococcal nuclease treatment of chromatin). Almost all nuclear DNA polymerase activity in lymphocytes was found to be associated with nucleosomes. The nucleosome-associated enzyme was mainly DNA polymerase beta in chromatin from resting and mainly DNA polymerase alpha in chromatin from concanavalin-A-stimulated lymphocytes.

摘要

在小鼠腹水细胞染色质中，超过一半的DNA聚合酶β被发现与单体核小体颗粒（通过微球菌核酸酶处理染色质产生）相关联。在淋巴细胞中，几乎所有的核DNA聚合酶活性都被发现与核小体相关。在静息淋巴细胞染色质中，与核小体相关的酶主要是DNA聚合酶β，而在伴刀豆球蛋白A刺激的淋巴细胞染色质中，主要是DNA聚合酶α。