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一种来自小鼠细胞的单链特异性DNA结合蛋白，可刺激DNA聚合酶。

A single-strand-specific DNA-binding protein from mouse cells that stimulates DNA polymerase.

作者信息

Otto B, Baynes M, Knippers R

出版信息

Eur J Biochem. 1977 Feb 15;73(1):17-24. doi: 10.1111/j.1432-1033.1977.tb11287.x.

DOI:10.1111/j.1432-1033.1977.tb11287.x
PMID:837935
Abstract

A protein of 30000-35000 molecular weight was isolated from mouse ascites cells. This protein binds preferentially to single-stranded DNA. Evidence is presented that this protein maintains single-stranded DNA in an extended configuration. In the presence of single-stranded template the protein stimulates mammalian DNA polymerase alpha but not the mammalian DNA polymerase beta and not some microbial DNA polymerases. The protein is phosphorylated in vitro by a chromatin-associated protein kinase. The modified DNA-binding protein does not stimulate the DNA polymerase alpha.

摘要

从小鼠腹水细胞中分离出一种分子量为30000 - 35000的蛋白质。这种蛋白质优先结合单链DNA。有证据表明，这种蛋白质能使单链DNA保持伸展构型。在单链模板存在的情况下，该蛋白质能刺激哺乳动物DNA聚合酶α，但不刺激哺乳动物DNA聚合酶β，也不刺激某些微生物DNA聚合酶。该蛋白质在体外可被一种与染色质相关的蛋白激酶磷酸化。经修饰的DNA结合蛋白不能刺激DNA聚合酶α。

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Recognition of chemical carcinogen-modified DNA by a DNA-binding protein.
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Proc Natl Acad Sci U S A. 1980 Jun;77(6):3201-5. doi: 10.1073/pnas.77.6.3201.
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EMBO J. 1982;1(12):1513-9. doi: 10.1002/j.1460-2075.1982.tb01348.x.
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