Woods J W, Woodward M P, Roth T F
J Cell Sci. 1978 Apr;30:87-97. doi: 10.1242/jcs.30.1.87.
Coated vesicles were purified and characterized from porcine brain and chicken oocyte. Electrophoresis on sodium dodecylsulphate (SDS) gels showed that coated vesicles from either source have three major proteins in common with apparent molecular weights of 180000, 120000, and 55000 Daltons. Negatively stained specimens from both sources appear to consist of a highly ordered array of short interconnected rods or ridges of material on the exterior surface of a membrane vesicle. Coated vesicles purified from porcine brain and chicken oocyte have mean external diameters of 75.0 and 85.0 nm, respectively. In coated vesicles of the appropriate orientation, portions of the coat material appear to be organized into hexagons and pentagons. Based on the observed variability of size and apparent structure, it is postulated that the basic structural subunits of coated vesicles are the short rods or ridges of material observed on the exterior membrane vesicle surface. It is suggested that multiples of the subunits can be assembled into many arrangements to yield coated vesicles of different sizes and coat structure. It is also proposed that the structural subunit is a complex of 3 proteins of molecular weight of 180000, 120000 and 55000 Daltons.
从猪脑和鸡卵母细胞中纯化并鉴定了被膜小泡。在十二烷基硫酸钠（SDS）凝胶上进行电泳显示，来自任一来源的被膜小泡都有三种主要蛋白质，其表观分子量分别为180000、120000和55000道尔顿。来自这两个来源的经负染色的标本似乎由膜小泡外表面上高度有序排列的短的相互连接的杆状或脊状物质组成。从猪脑和鸡卵母细胞中纯化的被膜小泡的平均外径分别为75.0和85.0纳米。在具有适当取向的被膜小泡中，部分被膜物质似乎被组织成六边形和五边形。基于观察到的大小和表观结构的变异性，推测被膜小泡的基本结构亚基是在外膜小泡表面观察到的短杆状或脊状物质。有人提出，亚基的倍数可以组装成多种排列方式，以产生不同大小和被膜结构的被膜小泡。还提出结构亚基是分子量为180000、120000和55000道尔顿的三种蛋白质的复合物。
