痛风患者培养的皮肤成纤维细胞嘌呤代谢的变化。
Variations in purine metabolism of cultured skin fibroblasts from patients with gout.
作者信息
Henderson J F, Rosenbloom F M, Kelley W N, Seegmiller J E
出版信息
J Clin Invest. 1968 Jul;47(7):1511-6. doi: 10.1172/JCI105844.
Abstract
Purine metabolism was studied in fibroblasts cultured from three patients with gout in an attempt to determine the biochemical bases of their disease. The rate of purine biosynthesis de novo was normal in one line of cells, but the rate of catabolism of adenine nucleotides to hypoxanthine and inosine was greatly increased. The rate of purine biosynthesis de novo was increased in two lines of cells, and this was associated with increased concentrations of 5-phosphoribosyl 1-pyrophosphate. Purine synthesis was also less sensitive than normal to feedback inhibition. The catabolism of inosinate synthesized de novo was increased.
摘要
对三名痛风患者培养的成纤维细胞中的嘌呤代谢进行了研究，以确定其疾病的生化基础。在一组细胞中，嘌呤从头合成的速率正常，但腺嘌呤核苷酸分解代谢为次黄嘌呤和肌苷的速率大大增加。在两组细胞中，嘌呤从头合成的速率增加，这与5-磷酸核糖1-焦磷酸浓度的增加有关。嘌呤合成对反馈抑制的敏感性也低于正常水平。从头合成的肌苷酸的分解代谢增加。
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