Chu M L, Jernigan H M, Fried M
Biochim Biophys Acta. 1978 Apr 27;518(2):298-307. doi: 10.1016/0005-2787(78)90186-7.
The quantity and activities of membrane-bound and free polysomes in livers from chick embryos at successive stages of development were compared in cell-free protein-synthesizing systems. Membrane-bound polysomes increased 2-fold between 8 and 18 days of development, while total ribosome content remained constant. Free polysome activity also remained constant during this period, while that of membrane-bound (total--free) polysomes decreased, possibly because of an increase in ribonuclease activity in this fraction. Serum albumin biosynthesis occurred primarily on membrane-bound polysomes. With liver development, increased secretion of serum proteins may be correlated with synthesis of serum albumin on increasing numbers of membrane bound polyribosomes.
在无细胞蛋白质合成系统中，比较了发育连续阶段的鸡胚肝脏中膜结合多核糖体和游离多核糖体的数量及活性。在发育的第8天到18天之间，膜结合多核糖体增加了2倍，而核糖体总含量保持不变。在此期间，游离多核糖体的活性也保持不变，而膜结合（总 - 游离）多核糖体的活性下降，这可能是因为该部分中核糖核酸酶活性增加。血清白蛋白的生物合成主要发生在膜结合多核糖体上。随着肝脏的发育，血清蛋白分泌增加可能与越来越多的膜结合多核糖体上血清白蛋白的合成有关。
