Morrow J, Stocco D, Barron E
J Cell Physiol. 1978 Jul;96(1):81-6. doi: 10.1002/jcp.1040960110.
The mutation rate to thioguanine resistance was 3.11 X 10(-6) in a near diploid V79 hamster cell line and 7.58 X 10(-8) in a near tetraploid derivative produced with colchicine. The specific activities of glucose-6-phosphate dehydrogenase and phosphoglycerate kinase of the tetraploid line were greater than that of the diploid which suggests that twice the number of active X chromosomes were present in the tetraploid. These results are compatible with the hypothesis that spontaneous variants resistant to thioguanine arise through mutation and chromosomal segregation, as has been suggested for induced mutations in tetraploid hamster cells.
在一个近二倍体V79仓鼠细胞系中，对硫代鸟嘌呤耐药的突变率为3.11×10⁻⁶，在用秋水仙碱产生的近四倍体衍生物中为7.58×10⁻⁸。四倍体系的葡萄糖-6-磷酸脱氢酶和磷酸甘油酸激酶的比活性高于二倍体，这表明四倍体中存在两倍数量的活性X染色体。这些结果与以下假设相符，即对硫代鸟嘌呤耐药的自发变体是通过突变和染色体分离产生的，正如在四倍体仓鼠细胞中的诱导突变所表明的那样。
