Slamenová D, Dusinská M, Nádaská M
Neoplasma. 1984;31(3):339-46.
The capacity of ftorafur to induce resistance to 6-thioguanine in V79 hamster cells was investigated. The treatment conditions were arranged in order to favor the induction of such gene mutations. The synchronous cells were treated in the beginning of S phase, i.e., when the replication of the genes responsible for the expression of resistance to 6-thioguanine took place. Part of the experiments was carried out in presence of the S9 microsomal fraction. The results showed that ftorafur was a weak mutagen after a short-term exposure in the presence of adequate amounts of the S9 fraction. In absence of the S9 fraction or when other than-optimal amounts were used, we repeatedly failed to detect any increase in the occurrence of mutated cells. Also the cytotoxic effects of this substance were investigated. We found that a short-term treatment of V79 hamster cells with lower concentrations of ftorafur resulted in decrease of growth rate, higher concentrations after a short-term treatment were in turn slightly toxic. Long-term treatments are, depending on the concentration and time of treatment used, considerably toxic.
研究了呋喃氟尿嘧啶诱导V79仓鼠细胞对6-硫鸟嘌呤产生抗性的能力。安排处理条件以利于此类基因突变的诱导。同步细胞在S期开始时进行处理，即负责表达对6-硫鸟嘌呤抗性的基因进行复制时。部分实验在S9微粒体组分存在的情况下进行。结果表明，在存在适量S9组分的情况下短期暴露后，呋喃氟尿嘧啶是一种弱诱变剂。在不存在S9组分或使用非最佳量时，我们多次未能检测到突变细胞发生率的任何增加。还研究了该物质的细胞毒性作用。我们发现，用较低浓度的呋喃氟尿嘧啶对V79仓鼠细胞进行短期处理会导致生长速率降低，短期处理后较高浓度则有轻微毒性。根据所用处理的浓度和时间，长期处理具有相当大的毒性。
