多头绒泡菌孢子中的核糖体DNA。

Ribosomal DNA in spores of Physarum polycephalum.

作者信息

Affolter H U, Braun R

出版信息

Biochim Biophys Acta. 1978 Jun 22;519(1):118-24. doi: 10.1016/0005-2787(78)90066-7.

Abstract

DNA was isolated from plasmodia, spores and newly hatched amoebae of the slime mould Physarum polycephalum. The DNA preparations were fractionated in CsCl gradients and each fraction hybridised to combined 19 S + 26 S rRNA. In all three DNA preparations hybridisation was found to be limited to satellite DNA (rho = 1.714 g/cm3) and at saturation was found to reach a level of 0.16--0.18 % of total DNA. The main band of nuclear DNA (rho = 1.702 g/cm3) did not hybridise appreciably. Further experiments using analytical CsCl gradients revealed that the ratio of satellite to main band DNA was similar in all three preparations. It is concluded that the genes for ribosomal RNA are equally reiterated in spores, hatching amoebae and in plasmodia. They appear to be similarly organised in all stages of the life cycle examined so far.

摘要

从多头绒泡菌的疟原虫、孢子和新孵化的变形虫中分离出DNA。将DNA制剂在CsCl梯度中分级分离，每个级分与19S + 26S rRNA混合物杂交。在所有三种DNA制剂中，发现杂交仅限于卫星DNA（ρ = 1.714 g/cm3），饱和时达到总DNA的0.16 - 0.18%。核DNA的主带（ρ = 1.702 g/cm3）没有明显杂交。使用分析性CsCl梯度的进一步实验表明，所有三种制剂中卫星DNA与主带DNA的比例相似。结论是，核糖体RNA基因在孢子、孵化的变形虫和疟原虫中同样存在重复。在迄今为止所研究的生命周期的所有阶段，它们的组织方式似乎相似。