多头绒泡菌核糖体RNA基因的组织

Organisation of genes for ribosomal RNA in Physarum polycephalum.

作者信息

Molgaard H V, Matthews H R, Bradbury E M

出版信息

Eur J Biochem. 1976 Sep 15;68(2):541-9. doi: 10.1111/j.1432-1033.1976.tb10842.x.

DOI:10.1111/j.1432-1033.1976.tb10842.x

Abstract

Physarum polycephalum nucleolar satellite DNA has been analysed by restriction enzyme digests and hybridisation to ribosomal RNA. The nucleolar DNA is isolated as molecules of molecular weight 39 X 10(6). The positions of sites of endo R-EcoRI and endo R-HindIII digestion have been determined accurately in the whole molecule. The endo R-EcoRI sites and one endo R-HindIII site are within the26-S rRNA complementary sequence and the other endo R-HindIII site is within the 19-S rRNA complementary sequence. The sites are arranged symmetrically about the centre of the molecule as if it were a palindrome.

摘要

多头绒泡菌核仁卫星DNA已通过限制性内切酶消化和与核糖体RNA杂交进行了分析。核仁DNA被分离为分子量为39×10⁶的分子。内切酶EcoRI和内切酶HindIII消化位点在整个分子中的位置已被精确确定。内切酶EcoRI位点和一个内切酶HindIII位点位于26 - S rRNA互补序列内，另一个内切酶HindIII位点位于19 - S rRNA互补序列内。这些位点围绕分子中心对称排列，就好像它是一个回文结构。