Ichimura K
Folia Biol (Praha). 1978;24(3):162-72.
The effect of chlorpromazine at concentrations from 10(-7) to 5 X 10(-5)M on the in vitro plaque-forming cell responses to sheep erythrocytes was tested. If CPZ was present in cultures during the whole incubation period, concentrations of CPZ more than 5 X 10(-6)M inhibited the PFC responses, whereas concentrations between 5 X 10(-7) and 5 X 10(-6)M had a slightly enhancing effect. If a defined concentration of CPZ (1.3 or 1.5 X 10(-5)M) was added to cultures for a brief period of time (12 or 24 h) at various intervals after the initiation of culture and antigenic stimulation, PFC responses were inhibited most effectively during the first brief periods of incubation. The later the drug was added, the more the extent of inhibition decreased. On the other hand, CPZ had no effect or a slightly enhancing effect when added in the very late period (72--95 h) of incubation. These findings suggest that the observed immunosuppression could be attributed mainly to the impairment of early cellular events, presumably cell interaction events, preceding the process of DNA synthesis and cell proliferation of antibody-forming cells and their precursors.
测试了浓度为10(-7)至5×10(-5)M的氯丙嗪对体外针对绵羊红细胞的空斑形成细胞反应的影响。如果在整个孵育期培养物中存在氯丙嗪，氯丙嗪浓度超过5×10(-6)M会抑制空斑形成细胞反应，而浓度在5×10(-7)至5×10(-6)M之间则有轻微增强作用。如果在培养和抗原刺激开始后的不同时间间隔向培养物中短暂添加确定浓度的氯丙嗪（1.3或1.5×10(-5)M）12或24小时，在孵育的最初短暂时期空斑形成细胞反应受到的抑制最有效。添加药物的时间越晚，抑制程度下降得越多。另一方面，在孵育的很晚时期（72 - 95小时）添加氯丙嗪则没有作用或有轻微增强作用。这些发现表明，观察到的免疫抑制可能主要归因于早期细胞事件的损害，大概是在抗体形成细胞及其前体的DNA合成和细胞增殖过程之前的细胞相互作用事件。
