纯化的125-I标记胰岛素抗体的制备及其性质

Miles L E, Hales C N

Biochem J. 1968 Jul;108(4):611-8. doi: 10.1042/bj1080611.

A procedure is described for the preparation of an insulin-immunoadsorbent containing 105-185mg. of insulin/g. of matrix, and with an antibody-binding capacity of 660mg./g. of insulin-immunoadsorbent. 2. Purified non-precipitating (125)I-labelled antibodies were prepared by iodination of the antibodies while they were isolated on the insulin-immunoadsorbent in order to protect at least one antigen-binding site. 3. The radioactive antibody was simply and rapidly separated from other radioactive material and damaging reagents, and found to be up to 94% pure as judged by its reaction with insulin-immunoadsorbent and unfixed insulin. 4. The (125)I-labelled antibody preparations had iodine contents of 0.5-64atoms/mol. of protein and specific radioactivities of 0.3-125mc/mg. of protein. 5. It is suggested that this is a simple method of producing a useful reagent.

描述了一种制备胰岛素免疫吸附剂的方法，该吸附剂每克基质含105 - 185毫克胰岛素，每克胰岛素免疫吸附剂的抗体结合能力为660毫克。2. 通过在胰岛素免疫吸附剂上分离抗体时对其进行碘化来制备纯化的非沉淀性（125）I标记抗体，以保护至少一个抗原结合位点。3. 放射性抗体可简单快速地与其他放射性物质和有害试剂分离，通过其与胰岛素免疫吸附剂和未固定胰岛素的反应判断，其纯度高达94%。4. （125）I标记抗体制剂的碘含量为每摩尔蛋白质0.5 - 64个原子，比放射性为每毫克蛋白质0.3 - 125微居里。5. 有人认为这是一种生产有用试剂的简单方法。

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核心技术专利：CN118964589B侵权必究

Miles L E, Hales C N

Biochem J. 1968 Jul;108(4):611-8. doi: 10.1042/bj1080611.

A procedure is described for the preparation of an insulin-immunoadsorbent containing 105-185mg. of insulin/g. of matrix, and with an antibody-binding capacity of 660mg./g. of insulin-immunoadsorbent. 2. Purified non-precipitating (125)I-labelled antibodies were prepared by iodination of the antibodies while they were isolated on the insulin-immunoadsorbent in order to protect at least one antigen-binding site. 3. The radioactive antibody was simply and rapidly separated from other radioactive material and damaging reagents, and found to be up to 94% pure as judged by its reaction with insulin-immunoadsorbent and unfixed insulin. 4. The (125)I-labelled antibody preparations had iodine contents of 0.5-64atoms/mol. of protein and specific radioactivities of 0.3-125mc/mg. of protein. 5. It is suggested that this is a simple method of producing a useful reagent.

描述了一种制备胰岛素免疫吸附剂的方法，该吸附剂每克基质含105 - 185毫克胰岛素，每克胰岛素免疫吸附剂的抗体结合能力为660毫克。2. 通过在胰岛素免疫吸附剂上分离抗体时对其进行碘化来制备纯化的非沉淀性（125）I标记抗体，以保护至少一个抗原结合位点。3. 放射性抗体可简单快速地与其他放射性物质和有害试剂分离，通过其与胰岛素免疫吸附剂和未固定胰岛素的反应判断，其纯度高达94%。4. （125）I标记抗体制剂的碘含量为每摩尔蛋白质0.5 - 64个原子，比放射性为每毫克蛋白质0.3 - 125微居里。5. 有人认为这是一种生产有用试剂的简单方法。