大鼠肝脏和肾脏线粒体中的两种天冬氨酸氨基转移酶形式。

Two forms of aspartate aminotransferase in rat liver and kidney mitochondria.

Bhargava M M, Sreenivasan A

Biochem J. 1968 Jul;108(4):619-24. doi: 10.1042/bj1080619.

Abstract

Butan-1-ol solubilizes that portion of rat liver mitochondrial aspartate aminotransferase (EC 2.6.1.1) that cannot be solubilized by ultrasonics and other treatments. 2. A difference in electrophoretic mobilities, chromatographic behaviour and solubility characteristics between the enzymes solubilized by ultrasonic treatment and by butan-1-ol was observed, suggesting the occurrence of two forms of this enzyme in rat liver mitochondria. 3. Half the aspartate aminotransferase activity of rat kidney homogenate was present in a high-speed supernatant fraction, the remainder being in the mitochondria. 4. A considerable increase in aspartate aminotransferase activity was observed when kidney mitochondrial suspensions were treated with ultrasonics or detergents. 5. All the activity after maximum activation was recoverable in the supernatant after centrifugation at 105000g for 1hr. 6. The electrophoretic mobility of the kidney mitochondrial enzyme was cathodic and that of the supernatant enzyme anodic. 7. Cortisone administration increased the activities of both mitochondrial and supernatant aspartate aminotransferases of liver, but only that of the supernatant enzyme of kidney.

摘要

丁醇可溶解大鼠肝线粒体天冬氨酸氨基转移酶（EC 2.6.1.1）中不能被超声及其他处理溶解的部分。2. 观察到经超声处理和丁醇溶解的酶在电泳迁移率、色谱行为及溶解性特征方面存在差异，这表明大鼠肝线粒体中存在该酶的两种形式。3. 大鼠肾匀浆中天冬氨酸氨基转移酶活性的一半存在于高速上清液部分，其余存在于线粒体中。4. 当肾线粒体悬液用超声或去污剂处理时，观察到天冬氨酸氨基转移酶活性显著增加。5. 最大激活后所有活性在105000g离心1小时后的上清液中均可回收。6. 肾线粒体酶的电泳迁移率为阴极，而上清液酶的电泳迁移率为阳极。7. 给予可的松可增加肝线粒体和上清液中天冬氨酸氨基转移酶的活性，但仅增加肾上清液酶的活性。