Garcia Mendoza C, Garcia Lopez M D, Uruburu F, Villanueva J R
J Bacteriol. 1968 Jun;95(6):2393-8. doi: 10.1128/jb.95.6.2393-2398.1968.
Cell membranes of the yeast Candida utilis isolated by lysis of protoplasts have been shown to be lipoprotein in nature. Electron microscopy shows that Mg(++) is responsible for maintaining the integrity of the membrane. A close serological relationship was found between membranes and cell walls isolated from the yeast. This relationship was exhibited not only by membranes obtained by strepzyme treatment but also by those obtained from the action of helicase enzyme. No such relationship existed between membranes and whole cells. Related data have been obtained by treatment of yeasts with different digestive enzymes. All of the results suggest that the protoplast membrane possesses traces of structural cell wall material. This material is detectable by serological tests, but not by electron microscopy.
通过原生质体裂解分离得到的产朊假丝酵母细胞膜已被证明本质上是脂蛋白。电子显微镜显示Mg(++)负责维持膜的完整性。发现从酵母中分离出的细胞膜与细胞壁之间存在密切的血清学关系。这种关系不仅在链霉蛋白酶处理获得的膜中表现出来，而且在解旋酶作用获得的膜中也表现出来。细胞膜与完整细胞之间不存在这种关系。通过用不同的消化酶处理酵母获得了相关数据。所有结果表明原生质体膜含有微量的结构细胞壁物质。这种物质可以通过血清学检测到，但不能通过电子显微镜检测到。
