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[感染鸡败血支原体的培养鸡胚细胞中核酸代谢的研究]

[Studies of nucleic acid metabolism in cultured chick embryo cells infected with Mycoplasma gallisepticum].

作者信息

Zakharian R A, Gasparian G G

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1978 Jun(6):71-5.

PMID:566996
Abstract

M. gallisepticum infection of cultured chick embryo cells led to a sharp reduction the rate of 3H-thymidine and 3H-uridine incorporation into DNA and RNA cells, and almost completely suppressed the transposition of uridine label from the nucleus into the cytoplasm, this pointing to the inhibition of escape of RNA synthesized de novo into the infected cells cytoplasm. As suggested, weak labeling of the cytoplasm after prolonged (about several hours) incubation of cultured cells with labeled urine could indicate infection of cell cultures with the mycoplasmae.

摘要

鸡胚细胞培养物感染鸡毒支原体导致3H-胸苷和3H-尿苷掺入DNA和RNA细胞的速率急剧降低,并且几乎完全抑制了尿苷标记从细胞核向细胞质的转运,这表明新合成的RNA向受感染细胞细胞质的逸出受到抑制。如前所述,用标记尿长时间(约数小时)培养细胞后细胞质标记较弱,这可能表明细胞培养物受到支原体感染。

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[Studies of nucleic acid metabolism in cultured chick embryo cells infected with Mycoplasma gallisepticum].[感染鸡败血支原体的培养鸡胚细胞中核酸代谢的研究]
Zh Mikrobiol Epidemiol Immunobiol. 1978 Jun(6):71-5.
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Nucleotide pools in Novikoff rat hepatoma cells growing in suspension culture. 3. Effects of nucleosides in medium on levels of nucleotides in separate nucleotide pools for nuclear and cytoplasmic RNA synthesis.悬浮培养的诺维科夫大鼠肝癌细胞中的核苷酸库。3. 培养基中核苷对用于核和细胞质RNA合成的单独核苷酸库中核苷酸水平的影响。
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