Markley J L, Putter I, Jardetzky O
Science. 1968 Sep 20;161(3847):1249-51. doi: 10.1126/science.161.3847.1249.
An analog of staphylococcal nuclease has been prepared in which all amino acids, except the six following, are fully deuterated: tryptophan; methionine; tyrosine, in ring positions 2 and 6; histidine, in ring position 2; aspartic acid and asparagine, beta-methylene; and glutamic acid and glutamine, gamma-methylene. The analog has a much simpler high-resolution nuclear magnetic resonance spectrum than the fully protonated enzyme. The effects of calcium ion and of the inhibitor 3', 5'-thymidine diphosphate on the spectrum of the analog were readily detected.
已制备出一种葡萄球菌核酸酶类似物，其中除以下六种氨基酸外，所有氨基酸均被完全氘代：色氨酸；甲硫氨酸；酪氨酸（在第2和6位环位置）；组氨酸（在第2位环位置）；天冬氨酸和天冬酰胺的β-亚甲基；以及谷氨酸和谷氨酰胺的γ-亚甲基。该类似物的高分辨率核磁共振谱比完全质子化的酶简单得多。钙离子和抑制剂3',5'-胸腺嘧啶二磷酸对该类似物谱的影响很容易检测到。
