固态核磁共振与膜蛋白。
Solid-state NMR and membrane proteins.
作者信息
Opella Stanley J
机构信息
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA.
出版信息
J Magn Reson. 2015 Apr;253:129-37. doi: 10.1016/j.jmr.2014.11.015. Epub 2014 Dec 29.
Abstract
The native environment for a membrane protein is a phospholipid bilayer. Because the protein is immobilized on NMR timescales by the interactions within a bilayer membrane, solid-state NMR methods are essential to obtain high-resolution spectra. Approaches have been developed for both unoriented and oriented samples, however, they all rest on the foundation of the most fundamental aspects of solid-state NMR, and the chemical shift and homo- and hetero-nuclear dipole-dipole interactions. Solid-state NMR has advanced sufficiently to enable the structures of membrane proteins to be determined under near-native conditions in phospholipid bilayers.
摘要
膜蛋白的天然环境是磷脂双分子层。由于该蛋白在核磁共振时间尺度上通过双层膜内的相互作用而固定，因此固态核磁共振方法对于获得高分辨率光谱至关重要。已针对未取向和取向样品开发了相关方法，然而，它们都基于固态核磁共振最基本的方面，即化学位移以及同核和异核偶极-偶极相互作用。固态核磁共振技术已经取得了长足的进步，能够在接近天然的条件下，在磷脂双分子层中确定膜蛋白的结构。
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Prog Nucl Magn Reson Spectrosc. 2014 Oct;82:1-26. doi: 10.1016/j.pnmrs.2014.07.001. Epub 2014 Jul 26.
2
Dynamic nuclear polarization-enhanced NMR on aligned lipid bilayers at ambient temperature.
J Am Chem Soc. 2014 Nov 5;136(44):15533-6. doi: 10.1021/ja509799s. Epub 2014 Oct 24.
3
De novo 3D structure determination from sub-milligram protein samples by solid-state 100 kHz MAS NMR spectroscopy.
Angew Chem Int Ed Engl. 2014 Nov 3;53(45):12253-6. doi: 10.1002/anie.201405730. Epub 2014 Sep 15.
4
Structure of the membrane protein MerF, a bacterial mercury transporter, improved by the inclusion of chemical shift anisotropy constraints.
J Biomol NMR. 2014 Sep;60(1):67-71. doi: 10.1007/s10858-014-9852-0. Epub 2014 Aug 8.
5
Rapid proton-detected NMR assignment for proteins with fast magic angle spinning.
J Am Chem Soc. 2014 Sep 3;136(35):12489-97. doi: 10.1021/ja507382j. Epub 2014 Aug 18.
6
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J Phys Chem B. 2014 Aug 14;118(32):9604-12. doi: 10.1021/jp505412j. Epub 2014 Jul 31.
7
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Q Rev Biophys. 2014 Aug;47(3):249-83. doi: 10.1017/S0033583514000080. Epub 2014 Jul 17.
8
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9
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