过氧化氢酶-过氧化氢系统。在无底物情况下亚基在细菌过氧化氢酶热失活中的作用。
The catalase-hydrogen peroxide system. Role of sub-units in the thermal deactivation of bacterial catalase in the absence of substrate.
作者信息
Jones P, Suggett A
出版信息
Biochem J. 1968 Aug;108(5):833-8. doi: 10.1042/bj1080833.
Abstract
- Kinetic studies of the thermal deactivation of bacterial catalase in the absence of substrate suggest that the reaction involves a protonation-induced reversible dissociation of catalase into catalatically inactive sub-units, followed by an irreversible transformation of the sub-units into deactivated products. It is possible that the sub-units are mono-haem species. The rate of deactivation decreases with increasing pressure in accordance with the predictions of the proposed model. 2. The results also imply that the addition of hydrogen peroxide substrate induces the re-formation of active catalase. Under appropriate conditions the activity of catalase is found to increase with time in a manner that is quantitatively consistent with the results of deactivation studies.
摘要
- 在没有底物的情况下，对细菌过氧化氢酶热失活的动力学研究表明，该反应涉及质子化诱导的过氧化氢酶可逆解离成无催化活性的亚基，随后亚基不可逆地转化为失活产物。亚基有可能是单血红素物种。根据所提出模型的预测，失活速率随压力增加而降低。2. 结果还表明，添加过氧化氢底物会诱导活性过氧化氢酶重新形成。在适当条件下，发现过氧化氢酶的活性随时间增加，其方式在数量上与失活研究结果一致。
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