de Vries W, Stouthamer A H
J Bacteriol. 1968 Aug;96(2):472-8. doi: 10.1128/jb.96.2.472-478.1968.
For six strains of Bifidobacterium bifidum (Lactobacillus bifidus), fermentation balances of glucose, lactose, galactose, mannitol, and xylose were determined. Products formed were acetate, l(+)-lactate, ethyl alcohol, and formate. l(+)-Lactate dehydrogenase of all strains studied was found to have an absolute requirement for fructose-1,6-diphosphate. The phosphoroclastic enzyme could not be demonstrated in cell-free extracts. Cell suspensions fermented pyruvate to equimolar amounts of acetate and formate. Alcohol dehydrogenase was shown in cell-free extracts. Possible explanations have been suggested for the differences in fermentation balances found for different strains and carbon sources. By enzyme determinations, it was shown that bifidobacteria convert mannitol to fructose-6-phosphate by an inducible polyol dehydrogenase and fructokinase. For one strain of B. bifidum, molar growth yields of glucose, lactose, galactose, and mannitol were determined. The mean value of Y (ATP), calculated from molar growth yields and fermentation balances, was 11.3.
对六株两歧双歧杆菌（双歧乳杆菌）测定了葡萄糖、乳糖、半乳糖、甘露醇和木糖的发酵平衡。生成的产物有乙酸、L(+)-乳酸、乙醇和甲酸。在所研究的所有菌株中，发现L(+)-乳酸脱氢酶对1,6-二磷酸果糖有绝对需求。在无细胞提取物中未检测到磷酸裂解酶。细胞悬液将丙酮酸发酵为等摩尔量的乙酸和甲酸。在无细胞提取物中检测到了乙醇脱氢酶。针对不同菌株和碳源发酵平衡的差异提出了可能的解释。通过酶测定表明，双歧杆菌通过诱导型多元醇脱氢酶和果糖激酶将甘露醇转化为6-磷酸果糖。对一株两歧双歧杆菌测定了葡萄糖、乳糖、半乳糖和甘露醇的摩尔生长产率。根据摩尔生长产率和发酵平衡计算得出的Y(ATP)平均值为11.3。
