Enzmann P J, Weiland F
Arch Virol. 1978;57(4):339-48. doi: 10.1007/BF01320073.
Hog cholera virus grown in PK-15 cells was purified by centrifugation through a sucrose cushion followed by sucrose gradient centrifugation. Analysis of virus labeled externally with [3H]sodium borohydride on polyacrylamide gel electrophoresis revealed two glycoproteins, gp55 and gp46. A third structural polypeptide, p36, seems not to be glycosylated. The gp46 was also found in the virus-free supernatant of infected cells. It could be demonstrated by radioimmune precipitation of virus labeled with[35S]methionine that all three polypeptides are specific for hog cholera virions. Electron microscopically hog cholera virus appeared as a spherical particle with a diameter of 42 +/- 8 nm. The virus particles frequently displayed a fringe of projections with a length of about 6--8 nm. The similarities of hog cholera virus with Alphaviruses and Flaviviruses are discussed.
在PK - 15细胞中培养的猪霍乱病毒，先通过蔗糖垫层离心，再经蔗糖梯度离心进行纯化。用[3H]硼氢化钠对病毒进行外部标记后，在聚丙烯酰胺凝胶电泳上分析发现有两种糖蛋白，即gp55和gp46。第三种结构多肽p36似乎未被糖基化。在受感染细胞的无病毒上清液中也发现了gp46。通过对用[35S]甲硫氨酸标记的病毒进行放射免疫沉淀可以证明，所有这三种多肽都是猪霍乱病毒粒子所特有的。电子显微镜下，猪霍乱病毒呈现为直径42 +/- 8纳米的球形颗粒。病毒粒子常常显示出长度约为6 - 8纳米的突起边缘。文中讨论了猪霍乱病毒与甲病毒属和黄病毒属病毒的相似性。
