猪霍乱病毒的结构成分。

The structural components of hog cholera virus.

作者信息

Enzmann P J, Rehberg H

出版信息

Z Naturforsch C Biosci. 1977 May-Jun;32(5-6):456-8. doi: 10.1515/znc-1977-5-627.

DOI:10.1515/znc-1977-5-627

Abstract

Hog cholera virus grown in PK-15 cells and SK-cells was labeled with [35S]methionine and [3H]uridine. At least 3 polypeptides were resolved by polyacrylamide gel electrophoresis after disruption of the virus with sodium dodecyl-sulfate. The molecular weights of the structural proteins were determined to be 55000 (p55), 46000 (gp46), and 36000 (p36). The molecular weight of the viral RNA was determined to be about 4 X 10(6) in polyacrylamide-agarose-gel electrophoresis. In sucrose gradients the RNA has a S20,w value of 40-45S.

摘要

在PK - 15细胞和SK细胞中培养的猪霍乱病毒用[35S]甲硫氨酸和[3H]尿苷进行标记。用十二烷基硫酸钠破坏病毒后，通过聚丙烯酰胺凝胶电泳至少分辨出3种多肽。结构蛋白的分子量测定为55000（p55）、46000（gp46）和36000（p36）。在聚丙烯酰胺 - 琼脂糖凝胶电泳中，病毒RNA的分子量测定约为4×10(6)。在蔗糖梯度中，RNA的S20,w值为40 - 45S。