Yonuschot G, Robey G, Mushrush G W, Helman D, Vande Woude G
Bioinorg Chem. 1978;8(5):397-404. doi: 10.1016/s0006-3061(00)80274-4.
This communication demonstrates further that terbium(III) can be used as a probe for DNA. The stoichiometry of terbium binding to DNA was measured by two new methods. In the first method, calf-thymus DNA was titrated with radioactive terbium-160, which is an isotope of the common terbium-159. The resulting DNA-terbium complex was trapped and measured on millipore filters. In the second method, a peak of UV absorption of terbium was found at 219 nm and was used to measure stocichiometry. By both methods, the stoichiometry of binding was one Tb(III) for each three available phosphate groups in DNA. Finally, a rapid method was developed using terbrium-160 to measure the amount of nucleic acid in a solution.
本通讯进一步证明铽（III）可作为DNA的探针。通过两种新方法测定了铽与DNA结合的化学计量。第一种方法是用放射性铽-160（普通铽-159的同位素）滴定小牛胸腺DNA。所得的DNA-铽复合物被捕获并在微孔滤膜上进行测量。第二种方法是在219nm处发现铽的紫外吸收峰，并用于测量化学计量。通过这两种方法，结合的化学计量是DNA中每三个可用磷酸基团对应一个Tb（III）。最后，开发了一种使用铽-160测量溶液中核酸量的快速方法。
