Yonuschot G, Helman D, Mushrush G, Vande Woude G, Robey G
Bioinorg Chem. 1978;8(5):405-18. doi: 10.1016/s0006-3061(00)80275-6.
This paper continues previous work on the analysis of nucleic acid-terbium complexes in the solid state. The fluorescence excitation and emission spectra of the RNA-terbium(III) complex is reported. The fluorescence excitation and emission spectra of both the RNA-terbium(III) and DNA-terbium(III) complexes as trapped on millipore filters is reported. One hundred percent of the DNA combined with terbium was trapped on millipore filters. Deoxyribonucleic acid was recovered from DNA-terbium(III) complexes trapped on millipore filters using SDS-extraction. Energy transfer was shown to occur from the bases in nucleic acids to the terbium ion, whereas the actual binding of terbium to nucleic acids was due to phosphate groups. The relative fluorescence of homopolyribonucleotide-terbium complexes showed that the guanine moiety was responsible for most of the observed fluorescence. Binding studies showed an equal affinity of radioactive terbium for all the homopolyribonucleotides. The fluorescence of solid-state DNA and RNA terbium complexes was used to measure picomole quantities of DNA or RNA.
本文延续了之前对固态核酸 - 铽配合物的分析工作。报道了RNA - 铽（III）配合物的荧光激发和发射光谱。还报道了捕获在微孔滤膜上的RNA - 铽（III）和DNA - 铽（III）配合物的荧光激发和发射光谱。与铽结合的DNA百分之百被捕获在微孔滤膜上。使用SDS萃取法从捕获在微孔滤膜上的DNA - 铽（III）配合物中回收脱氧核糖核酸。结果表明，能量从核酸中的碱基转移至铽离子，而铽与核酸的实际结合是由于磷酸基团。同聚核糖核苷酸 - 铽配合物的相对荧光表明，鸟嘌呤部分是观察到的大部分荧光的来源。结合研究表明，放射性铽对所有同聚核糖核苷酸具有相同的亲和力。固态DNA和RNA铽配合物的荧光被用于测量皮摩尔量的DNA或RNA。
