Knittle J L, Hirsch J
J Clin Invest. 1968 Sep;47(9):2091-8. doi: 10.1172/JCI105894.
The effect of infantile nutritional levels on adipose tissue cellularity and metabolism was studied in two groups of Sprague-Dawley rats. Caloric intake was varied during the suckling period by manipulating litter size immediately after birth; however, all animals had free access to food after weaning. The epididymal fat pads of animals raised in small litters were heavier than those of their paired siblings raised in large litters. Initially, the differences in pad weight were accounted for primarily by differences in total cell number; however, at 20 wk both cell number and cell size contributed equally. The rate of glucose incorporation into CO(2) and triglyceride during in vitro incubations was the same for both groups if expressed on a per cell basis; therefore total tissue incorporation was greater in animals with more cells. The results support the hypothesis that early nutritional experiences can effect permanent changes in the cell number and size of the epididymal fat depot and that total cell number is important in the total metabolism of this organ. These findings and the fact that extreme human obesity is accompanied by similar alterations in cellularity and metabolism indicate that early nutritional experiences should be studied further as a guide to the etiology of obesity in man.
在两组斯普拉格-道利大鼠中研究了婴儿营养水平对脂肪组织细胞构成和代谢的影响。通过在出生后立即控制窝仔数来改变哺乳期的热量摄入；然而，所有动物断奶后均可自由进食。在小窝中饲养的动物的附睾脂肪垫比在大窝中饲养的其配对同胞的附睾脂肪垫更重。最初，脂肪垫重量的差异主要由总细胞数的差异所致；然而，在20周时，细胞数和细胞大小的作用相同。如果以每个细胞为基础表示，两组在体外培养期间葡萄糖掺入二氧化碳和甘油三酯的速率相同；因此，细胞较多的动物的总组织掺入量更大。这些结果支持以下假设：早期营养经历可导致附睾脂肪库的细胞数量和大小发生永久性变化，并且总细胞数对该器官的总代谢很重要。这些发现以及极端人类肥胖伴有细胞构成和代谢的类似改变这一事实表明，应进一步研究早期营养经历，以作为人类肥胖病因学的指导。
