Tresner H D, Hayes J A, Backus E J
Appl Microbiol. 1968 Aug;16(8):1134-6. doi: 10.1128/am.16.8.1134-1136.1968.
A survey was made of the NaCl tolerance of approximately 1,300 Streptomyces strains belonging to 313 species. The growth medium of the organisms was supplemented with a graded series of NaCl concentrations (4, 7, 10, and 13%). Only 1.8% of the species could not tolerate 4% NaCl; 26.9% could grow at a maximum of 4%; 49.7% could tolerate a maximum of 7%; 18.8% could grow at a maximum of 10%; and only 2.8% could tolerate 13% NaCl. In evaluating the relationships of NaCl tolerance to various taxonomic features, higher tolerance was statistically associated with the "yellow" and possibly the "white"-spored streptomycetes, whereas the "red"-spored series tended to have lesser tolerance. Higher tolerance was also indicated for spiny-spored species, as a group, than for smooth-spored forms. Likewise, nonproducers of melanin, collectively, were more NaCl tolerant than melanin-producing species. Uniformity of test responses between strains of species studied suggested the usefulness of NaCl tolerance as a taxonomic criterion.
对属于313个物种的约1300株链霉菌菌株的耐氯化钠能力进行了调查。在生物体的生长培养基中添加了一系列梯度浓度的氯化钠（4%、7%、10%和13%）。只有1.8%的物种不能耐受4%的氯化钠；26.9%的物种在最高4%的氯化钠浓度下能够生长；49.7%的物种能够耐受最高7%的氯化钠；18.8%的物种在最高10%的氯化钠浓度下能够生长；只有2.8%的物种能够耐受13%的氯化钠。在评估耐氯化钠能力与各种分类学特征的关系时，较高的耐受性在统计学上与“黄色”以及可能的“白色”孢子链霉菌相关，而“红色”孢子系列的耐受性往往较低。作为一个群体，有刺孢子的物种比光滑孢子的物种表现出更高的耐受性。同样，总体而言，不产生黑色素的物种比产生黑色素的物种更耐氯化钠。所研究物种的菌株之间测试反应的一致性表明耐氯化钠能力作为一种分类标准是有用的。
