Otto L A, Deboo B S, Capers E L, Pickett M J
J Clin Microbiol. 1978 Apr;7(4):341-5. doi: 10.1128/jcm.7.4.341-345.1978.
Biochemical characteristics and antibiotic susceptibilities of five strains of Pseudomonas vesicularis isolated from cervical cultures are reported. The organisms were recovered from Thayer-Martin medium, which, because of its inhibitory properties, restricts over-growth by most other species. Our findings agree with those of Kaltenbach and associates (J. Clin. Microbiol., 1:339--344, 1975) on the importance of esculin hydrolysis, maltose oxidation, and pigmentation in the identification of P. vesicularis. Comparative carbohydrate oxidation studies showed agreement in three of four methods (oxidative attack, buffered single substrate, King oxidative/fermentative medium). No oxidation of carbohydrates was observed in commercial oxidative/fermentative medium. In addition to biochemical characteristics, antibiograms can be useful auxiliary aids in the identification of P. vesicularis and in its differentiation from P. diminuta, a closely related species.
本文报道了从宫颈培养物中分离出的五株泡囊假单胞菌的生化特性及抗生素敏感性。这些菌株从Thayer-Martin培养基中分离得到,该培养基因其抑制特性,可限制大多数其他菌种过度生长。我们的研究结果与Kaltenbach及其同事(《临床微生物学杂志》,1:339 - 344,1975年)关于七叶苷水解、麦芽糖氧化及色素沉着在泡囊假单胞菌鉴定中的重要性的研究结果一致。比较碳水化合物氧化研究表明,四种方法中的三种(氧化攻击法、缓冲单一底物法、King氧化/发酵培养基法)结果一致。在商业氧化/发酵培养基中未观察到碳水化合物氧化现象。除生化特性外,抗菌谱在泡囊假单胞菌的鉴定及其与密切相关的微小假单胞菌的鉴别中可为有用的辅助手段。