Kaltenbach C M, Moss C W, Weaver R E
J Clin Microbiol. 1975 Apr;1(4):339-44. doi: 10.1128/jcm.1.4.339-344.1975.
The cultural characteristics, biochemical activity, and cellular fatty acid composition of Pseudomonas diminuta and Pseudomonas vesiculare provided means for differentiation of these closely related species. Broth cultures of P. diminuta showed turbid growth and a distinct surface pellicle after 24 h at 35 C. P. vesiculare had no pellicle, and only light, diffuse growth was observed. All strains of P. vesiculare oxidized maltose and hydrolyzed esculin to varying degrees; P. diminuta was negative in these tests. The cellular fatty acids of these two species were similar, except that P. diminuta possessed a C19 cyclopropane acid which was not detected in P. vesiculare.
纤细假单胞菌和泡囊假单胞菌的培养特性、生化活性及细胞脂肪酸组成提供了区分这些近缘种的方法。纤细假单胞菌的肉汤培养物在35℃培养24小时后呈现浑浊生长并形成明显的表面菌膜。泡囊假单胞菌没有菌膜,仅观察到轻微的、弥漫性生长。所有泡囊假单胞菌菌株都能不同程度地氧化麦芽糖并水解七叶苷;纤细假单胞菌在这些试验中呈阴性。这两个种的细胞脂肪酸相似,只是纤细假单胞菌含有一种在泡囊假单胞菌中未检测到的C19环丙烷酸。