Feldherr C M
J Cell Biol. 1968 Oct;39(1):49-54. doi: 10.1083/jcb.39.1.49.
Colloidal gold was injected into the cytoplasm of amebae (Amoeba proteus) approximately 5 min, 1 hr, and 2 hr after cytokinesis. Later, interphase cells were similarly treated. All of the amebae were fixed about 50 min after injection and were examined with the electron microscope in order to determine the distribution of the gold. It was found that for a period of 2 hr after division the uptake of gold by the nuclei was significantly greater than that during late interphase. Correlation of the gold distribution with the morphology of the nuclear envelope indicated that an inverse relationship exists between the rate of incorporation of colloid into the nucleoplasm and the degree of reconstitution of the fibrous lamina ("honeycomb" structure). These data support the view that the fibrous lamina functions in regulating nucleocytoplasmic exchanges.
在胞质分裂后约5分钟、1小时和2小时，将胶体金注入变形虫（大变形虫）的细胞质中。之后，对间期细胞进行类似处理。所有变形虫在注射后约50分钟固定，并用电子显微镜检查以确定金的分布。发现分裂后2小时内，细胞核对金的摄取明显大于间期后期。金分布与核膜形态的相关性表明，胶体掺入核质的速率与纤维状层（“蜂窝”结构）的重建程度呈反比关系。这些数据支持了纤维状层在调节核质交换中起作用的观点。
