用微电极检测核膜的一些电学性质
SOME ELECTRICAL PROPERTIES OF A NUCLEAR MEMBRANE EXAMINED WITH A MICROELECTRODE.
作者信息
LOEWENSTEIN W R, KANNO Y
出版信息
J Gen Physiol. 1963 Jul;46(6):1123-40. doi: 10.1085/jgp.46.6.1123.
Abstract
Electrical potential and resistance were measured with microelectrodes in in situ and isolated nuclei of gland cells of Drosophila flavorepleta. The nucleus-cytoplasm boundary was found to be rather impermeable to ion diffusion. It presents a resistance of the order of 1 ohm cm(2) and sustains a "resting" potential, the nucleoplasm being about 15 mv negative with respect to the cytoplasm. Both the resistance and potential appear to be associated with the nuclear membrane: the potential declines to zero and the resistance to a fraction of its original value, when the membrane is perforated experimentally.
摘要
用电极对黄腹果蝇腺细胞的原位细胞核和分离细胞核进行了电位和电阻测量。发现细胞核与细胞质的边界对离子扩散相当不通透。它呈现出约1欧姆·厘米²的电阻，并维持一个“静息”电位，核质相对于细胞质约为负15毫伏。电阻和电位似乎都与核膜有关：当通过实验使膜穿孔时，电位降至零，电阻降至其原始值的一小部分。
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本文引用的文献
1
Some physical aspects of bioelectric phenomena.生物电现象的一些物理方面。
Proc Natl Acad Sci U S A. 1949 Oct;35(10):558-66. doi: 10.1073/pnas.35.10.558.
2
An analysis of the end-plate potential recorded with an intracellular electrode.用细胞内电极记录的终板电位分析。
J Physiol. 1951 Nov 28;115(3):320-70. doi: 10.1113/jphysiol.1951.sp004675.
3
Some electrical properties of the membrane of a cell nucleus.细胞核膜的一些电学特性。
Nature. 1962 Aug 4;195:462-4. doi: 10.1038/195462a0.
4
The fine structure of neurons.神经元的精细结构。
J Biophys Biochem Cytol. 1955 Jan;1(1):69-88. doi: 10.1083/jcb.1.1.69.
5
A STUDY OF THE NUCLEUS AND CELL MEMBRANES OF OOCYTES WITH AN INTRA-CELLULAR ELECTRODE.用细胞内电极对卵母细胞核与细胞膜的研究
Exp Cell Res. 1963 Jun;31:149-66. doi: 10.1016/0014-4827(63)90164-2.
6
The electrical conductance and potential across the membrane of some cell nuclei.某些细胞核膜的电导和电位。
J Cell Biol. 1963 Feb;16(2):421-5. doi: 10.1083/jcb.16.2.421.
7
Further observations on the nuclear envelope of the animal cell.关于动物细胞核膜的进一步观察。
J Biophys Biochem Cytol. 1959 Oct;6(2):147-56. doi: 10.1083/jcb.6.2.147.
8
The ultrastructure of cell membranes and their derivatives.细胞膜及其衍生物的超微结构。
Biochem Soc Symp. 1959;16:3-43.
9
The effect of internal and external potassium concentration on the membrane potential of frog muscle.细胞内外钾离子浓度对蛙肌膜电位的影响。
J Physiol. 1956 Sep 27;133(3):631-58. doi: 10.1113/jphysiol.1956.sp005615.
10
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J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):431-4. doi: 10.1083/jcb.2.4.431.
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