中链脂肪酰辅酶A合成酶的研究。酶组分II：反应机制及特异性
Studies on medium-chain fatty acyl-coenzyme a synthetase. Enzyme fraction II: mechanism of reaction and specific properties.
作者信息
Bar-Tana J, Rose G
出版信息
Biochem J. 1968 Sep;109(2):283-92. doi: 10.1042/bj1090283.
Abstract
- The mechanism of reaction of fatty acyl-CoA synthesis catalysed by fatty acyl-CoA synthetase from ox liver (fraction II; Bar-Tana, Rose & Shapiro, 1968) was investigated by a kinetic study of CoA disappearance dependent on butyrate plus ATP or butyryl-AMP (overall and partial reaction b respectively). 2. Contrary to findings with another enzyme (fraction I), a Bi Uni Uni Bi Ping Pong mechanism (Cleland, 1963a,b,c) corresponding to Berg's (1956) scheme of reaction was eliminated and an ordered Ter Ter mechanism with an A-C-B (standing for ATP, CoA and butyrate respectively) sequence of substrate entry for the overall reaction was established for fraction II. Partial reaction (b) was found to follow the ;Iso-Theorell-Chance' mechanism. 3. Also, in contrast with results obtained with fraction I, no allosteric properties could be demonstrated with fraction II.
摘要
- 通过对依赖丁酸盐加ATP或丁酰-AMP（分别为总反应和部分反应b）的辅酶A消失进行动力学研究，探究了牛肝脂肪酸酰基辅酶A合成酶（组分II；Bar-Tana、Rose和Shapiro，1968年）催化的脂肪酸酰基辅酶A合成反应机制。2. 与另一种酶（组分I）的研究结果相反，排除了与伯格（1956年）反应方案相对应的双单-单双乒乓机制（Cleland，1963a、b、c），并为组分II建立了总反应底物进入顺序为A-C-B（分别代表ATP、辅酶A和丁酸盐）的有序三三机制。发现部分反应（b）遵循“伊索-泰奥雷尔-钱斯”机制。3. 此外，与组分I的结果相反，未证明组分II具有变构性质。
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