来自大鼠肝脏的微粒体棕榈酰辅酶A合成酶。部分反应和交换反应。
Microsomal palmitoyl coenzyme A synthetase from rat liver. Partial and exchange reactions.
作者信息
Bar-Tana J, Rose G, Shapiro B
出版信息
Biochem J. 1972 Oct;129(5):1101-7. doi: 10.1042/bj1291101.
Abstract
The partial and exchange reactions of long-chain fatty acid activation were determined by using purified microsomal long-chain fatty acyl-CoA synthetase (EC 6.2.1.3). No significant ATP formation from palmitoyl-AMP and PP(i), nor palmitoyl-AMP-dependent CoA disappearance could be demonstrated. Similarly, no palmitate-dependent [(32)P(2)]PP(i)-ATP exchange was catalysed by the pure enzyme. The above partial and exchange reactions were, however, catalysed by the parent microsomal fraction at a rate similar to that of the overall reaction. The implications of these results are discussed.
摘要
通过使用纯化的微粒体长链脂肪酰辅酶A合成酶（EC 6.2.1.3）来测定长链脂肪酸活化的部分反应和交换反应。未证实棕榈酰-AMP和焦磷酸（PP(i)）能显著形成ATP，也未证实棕榈酰-AMP依赖性辅酶A的消失。同样，纯酶也未催化棕榈酸依赖性的[(32)P(2)]PP(i)-ATP交换反应。然而，上述部分反应和交换反应可由微粒体母液以与总反应相似的速率催化。讨论了这些结果的意义。
相似文献
1
Microsomal palmitoyl coenzyme A synthetase from rat liver. Partial and exchange reactions.
Biochem J. 1972 Oct;129(5):1101-7. doi: 10.1042/bj1291101.
2
Palmitoyl-coenzyme A synthetase. Mechanism of reaction.
Biochem J. 1973 Feb;131(2):199-209. doi: 10.1042/bj1310199.
3
Palmitoyl-coenzyme A synthetase. Isolation of an enzyme-bound intermediate.
Biochem J. 1973 Nov;135(3):411-6. doi: 10.1042/bj1350411.
4
Properties and substrate specificity of some reactions catalysed by a short-chain fatty acyl-coenzyme A synthetase from seeds of Pinus radiata.
Biochem J. 1974 Mar;137(3):423-33. doi: 10.1042/bj1370423.
5
The relationship between palmitoyl-coenzyme A synthetase activity and esterification of sn-glycerol 3-phosphate in rat liver mitochondria.
Biochem J. 1973 Apr;132(4):697-706. doi: 10.1042/bj1320697.
6
The purification and properties of microsomal palmitoyl-coenzyme A synthetase.
Biochem J. 1971 Apr;122(3):353-62. doi: 10.1042/bj1220353.
7
Acyl-Coenzyme A synthetase and fatty acid oxidation in rat liver peroxisomes.
J Biochem. 1978 Nov;84(5):1177-81. doi: 10.1093/oxfordjournals.jbchem.a132234.
8
Palmitate activation and esterification in microsomal fractions of rat liver.
Biochem J. 1975 Oct;152(1):39-49. doi: 10.1042/bj1520039.
9
Long-chain fatty acyl-CoA synthetase from rat liver microsomes. EC 6.2.1.3 fatty acyl-CoA ligase (ATP).
Methods Enzymol. 1975;35:117-22. doi: 10.1016/0076-6879(75)35146-x.
10
Long-chain acyl-coenzyme A synthetase from rat brain microsomes. Kinetic studies using [1-14C]docosahexaenoic acid substrate.
Eur J Biochem. 1984 Nov 15;145(1):21-9. doi: 10.1111/j.1432-1033.1984.tb08517.x.
引用本文的文献
1
Acyl coenzyme A synthetase from Pseudomonas fragi catalyzes the synthesis of adenosine 5'-polyphosphates and dinucleoside polyphosphates.
J Bacteriol. 1998 Jun;180(12):3152-8. doi: 10.1128/JB.180.12.3152-3158.1998.
2
Palmitoyl-coenzyme A synthetase. Isolation of an enzyme-bound intermediate.
Biochem J. 1973 Nov;135(3):411-6. doi: 10.1042/bj1350411.
3
Rat liver microsomal palmitoyl-coenzyme A synthetase. Structural properties.
Biochem J. 1973 Mar;131(3):443-9. doi: 10.1042/bj1310443.
4
Palmitoyl-coenzyme A synthetase. Mechanism of reaction.
Biochem J. 1973 Feb;131(2):199-209. doi: 10.1042/bj1310199.
5
The relationship between palmitoyl-coenzyme A synthetase activity and esterification of sn-glycerol 3-phosphate by the microsomal fraction of guinea-pig intestinal mucosa.
Biochem J. 1973 Apr;132(4):707-15. doi: 10.1042/bj1320707.
6
Properties and substrate specificity of some reactions catalysed by a short-chain fatty acyl-coenzyme A synthetase from seeds of Pinus radiata.
Biochem J. 1974 Mar;137(3):423-33. doi: 10.1042/bj1370423.
本文引用的文献
1
DITHIOTHREITOL, A NEW PROTECTIVE REAGENT FOR SH GROUPS.
Biochemistry. 1964 Apr;3:480-2. doi: 10.1021/bi00892a002.
2
The kinetics of enzyme-catalyzed reactions with two or more substrates or products. I. Nomenclature and rate equations.
Biochim Biophys Acta. 1963 Jan 8;67:104-37. doi: 10.1016/0006-3002(63)91800-6.
3
Role of magnesium in acetyl coenzyme A formation by acetothiokinase.
Science. 1959 Apr 3;129(3353):895-6. doi: 10.1126/science.129.3353.896.
4
Synthesis and properties of palmityl adenylate, palmityl coenzyme A, and palmityl glutathione.
Biochim Biophys Acta. 1958 Aug;29(2):263-9. doi: 10.1016/0006-3002(58)90183-5.
5
A relation between non-esterified fatty acids in plasma and the metabolism of glucose.
J Clin Invest. 1956 Feb;35(2):150-4. doi: 10.1172/JCI103259.
6
Studies of the formation and utilization of bound CoA in glyceride biosynthesis.
Biochim Biophys Acta. 1967 Aug 8;144(1):25-33. doi: 10.1016/0005-2760(67)90074-4.
7
A coenzyme A analogue, desulpho-coA; preparation and effects on various enzymes.
Biochem Biophys Res Commun. 1966 Apr 19;23(2):208-13. doi: 10.1016/0006-291x(66)90529-8.
8
Studies on medium-chain fatty acyl-coenzyme a synthetase. Enzyme fraction II: mechanism of reaction and specific properties.
Biochem J. 1968 Sep;109(2):283-92. doi: 10.1042/bj1090283.
9
Studies on medium-chain fatty acyl-coenzyme a synthetase. Enzyme fraction I: mechanism of reaction and allosteric properties.
Biochem J. 1968 Sep;109(2):275-82. doi: 10.1042/bj1090275.
10
Studies on medium-chain fatty acyl-coenzyme a synthetase. Purification and properties.
Biochem J. 1968 Sep;109(2):269-74. doi: 10.1042/bj1090269.
Zotero 插件