Masouredis S P, Sudora E J, Mahan L, Victoria E J
Transfusion. 1976 Mar-Apr;16(2):94-106. doi: 10.1046/j.1537-2995.1976.16276155121.x.
The ultrastructural distribution pattern and antigen site density of the major Rh antigens (C, c, E, e, and D) on red blood cell ghosts sensitized with WITH IgG Rh antibodies were determined using electron microscopy and ferritin conjugated rabbit anti-human IgG. The ferritin particle distribution pattern of all the Rh antigens studied was random and ranged from relatively isolated monodispersed clusters containing less than eight ferritin particles to large localized aggregates or masses of 20 or more ferritin particles. Evidence is presented to indicate that most of the antigen clustering was due to the conjugate during staining of the cell bound IgG on the stroma preparations. The conjugate-induced antigen clustering was influenced by both the titer of the conjugate and the staining time. Whether a similar degree of antigen mobility exists in the native membrane remains to be determined. Estimates of the number of cell bound IgG molecules (number of antigenic determinants) based on ferritin particle scoring of IgG Rh antibody sensitized red blood cells under nonsaturating conditions were in the range of 20,000 to 32,000 for each of the Rh antigens. These findings are consistent with the view that the Rh antigen complex is associated with a single membrane component.
使用电子显微镜和铁蛋白偶联兔抗人IgG，确定了用IgG Rh抗体致敏的红细胞血影上主要Rh抗原（C、c、E、e和D）的超微结构分布模式和抗原位点密度。所研究的所有Rh抗原的铁蛋白颗粒分布模式是随机的，范围从含有少于八个铁蛋白颗粒的相对孤立的单分散簇到含有20个或更多铁蛋白颗粒的大的局部聚集体或团块。有证据表明，大多数抗原聚集是由于在基质制剂上对细胞结合IgG进行染色期间的偶联物所致。偶联物诱导的抗原聚集受偶联物效价和染色时间的影响。天然膜中是否存在相似程度的抗原流动性仍有待确定。在非饱和条件下，基于对IgG Rh抗体致敏红细胞的铁蛋白颗粒评分，对每个Rh抗原的细胞结合IgG分子数量（抗原决定簇数量）的估计范围为20,000至32,000。这些发现与Rh抗原复合物与单一膜成分相关的观点一致。
