del Castillo J, Escalona de Motta G
J Cell Biol. 1978 Sep;78(3):782-4. doi: 10.1083/jcb.78.3.782.
The mechanical activity of frog sartorius muscle fibers can be uncoupled from the electrical activity of their surface membranes by immersing the preparation in Ringer solution containing either 1.5 or 2.0 M of formamide for 15--20 min. This uncoupling is not reversed when the muscle is transferred to normal frog Ringer solution. Formamide does not affect the electrical activity of the sciatic nerve branch, and both endplate potentials and miniature endplate potentials may be recorded from the uncoupled muscles. Prolonged exposure to formamide, beyond the time needed to paralyze, causes neuromuscular block.
将蛙缝匠肌纤维标本浸入含有1.5或2.0M甲酰胺的林格氏液中15 - 20分钟，可使其机械活动与其表面膜的电活动解偶联。当肌肉转移到正常蛙林格氏液中时，这种解偶联不会逆转。甲酰胺不影响坐骨神经分支的电活动，并且可以从未解偶联的肌肉中记录终板电位和微小终板电位。长时间暴露于甲酰胺，超过麻痹所需时间，会导致神经肌肉阻滞。
