Remy W, Bockendahl H, Stüttgen G, Petersen G
Acta Derm Venereol. 1976;56(1):43-6.
Following brief exposure of cryostat sections of human skin to the action of proteolytic enzymes (papain or trypsin), there was a pronounced increase in the antibody binding sites of the basement membrane zone, shown in indirect immunofluorescence by an increased intensity in fluorescence of the basement membrane zone as compared with preparations which had not previously undergone incubation, and by an increase of 5-6 (papain) or 4-5 (trypsin) titre dilution steps. This effect was practically absent when guinea-pig tongue was used as the antigenic substrate. In conjunction with findings published in the literature, our results can be interpreted as indicating that the activation of the basement membrane zone antigens by proteolytic enzymes is associated with an increase in antigenicity which results in the formation of "auto-antibodies" of the basement membrane zone antibody type.
在人体皮肤的低温恒温器切片短暂暴露于蛋白水解酶（木瓜蛋白酶或胰蛋白酶）的作用后，基底膜区的抗体结合位点显著增加，间接免疫荧光显示，与未预先孵育的标本相比，基底膜区荧光强度增加，且滴度稀释步骤增加了5 - 6（木瓜蛋白酶）或4 - 5（胰蛋白酶）。当用豚鼠舌头作为抗原底物时，这种效应实际上不存在。结合文献发表的研究结果，我们的结果可以解释为蛋白水解酶激活基底膜区抗原与抗原性增加有关，这导致了基底膜区抗体类型“自身抗体”的形成。
